Evidence map›Paper›PMID 32894203›Full record

ArticleStem cell research & therapy2020

hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β

Linlu Cui, Hongchu Bao, Zhongfeng Liu, Xuejing Man, Hongyuan Liu, Yun Hou, Qianqian Luo, Siyuan Wang, Qiang Fu, Hongqin Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
75citing papers in PubMed, 2 pooled it
10.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

75 citing papers in PubMed, 2 syntheses or guidelines pooled it, 110 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Multimodal ultrasound assessment of ovarian reserve.Abdominal radiology (New York) · 2026
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  14. Molecular mechanisms of ovarian fibrosis.Molecular human reproduction · 2026
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15 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Linlu CuiCollege of Basic Medicine & Institute of Reproductive Diseases, Binzhou Medical University, Yantai, 264003, Shandong, China.
Hongchu BaoDepartment of Clinical Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, China.
Zhongfeng LiuYantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, Shandong, China.
Xuejing ManDepartment of Clinical Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, China.
Hongyuan LiuClinical Medical School, Binzhou Medical University, Yantai, Shandong, China.
Yun HouCollege of Basic Medicine, Binzhou Medical University, Yantai, 264003, Shandong, China.
Qianqian LuoCollege of Basic Medicine, Binzhou Medical University, Yantai, 264003, Shandong, China.
Siyuan WangClinical Medical School, Binzhou Medical University, Yantai, Shandong, China.
Qiang FuSchool of pharmacy, Binzhou Medical University, Yantai, Shandong, China. qiangfu11@fudan.edu.cn.
Hongqin ZhangCollege of Basic Medicine & Institute of Reproductive Diseases, Binzhou Medical University, Yantai, 264003, Shandong, China. byzhhq@bzmc.edu.cn.ORCID 0000-0003-1440-7365
Binzhou Medical University · CNYuhuangding Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe basic pathological changes of primary ovarian insufficiency (POI) include ovarian tissue fibrosis and follicular development disorders. The human umbilical cord mesenchymal stem cell (hUMSC) transplantation has been shown an effective method to improve the ovarian function in POI rat model; however, the exact mechanisms are still unclear. The purpose of this study is to investigate whether the recovery of ovarian function in POI rats is related to the inhibition of tissue fibrosis following hUMSC transplantation. Furthermore, the transforming growth factor-β

methodsThe primary ovarian insufficiency (POI) rat model was established by intraperitoneal injection of chemotherapy drug cisplatin (CDDP) for 7 days. The levels of serum sex hormones were measured using enzyme-linked immunosorbent assay (ELISA). The tissue fibrosis in the ovary was examined using Masson staining and Sirius red staining. The collagen fibers in the ovarian tissues were detected by Western blot analysis. To investigate the mechanisms of ovarian function recovery following hUMSC transplantation, ovarian stromal cells were isolated from the ovarian cortex of immature rats. The expression of Cytochrome P450 17A1 (Cyp17a1) and fibrosis marker of alpha smooth muscle actin (α-SMA) in ovarian stromal cells was examined using immunofluorescence analysis. Also, the protein levels of Cyp17a1 and α-SMA in ovarian stromal cells were examined by Western blot analysis. The expression of TGF-β

resultsThe results show that the function of the ovary in POI rats was significantly improved after hUMSC transplantation. The expression of fibrosis markers (α-SMA) and production of Collagen Type I (Collagen I) and Collagen Type III (Collagen III) in POI rats were significantly inhibited in POI rats following hUMSC transplantation. In the cultured ovarian stromal cells, the decrease of TGF-β

conclusionOur study demonstrated that the TGF-β

Indexed as

Primary Ovarian InsufficiencySmad3 ProteinAnimalsCell DifferentiationFemaleFibrosisHumansRatsSignal TransductionStromal CellsTransforming Growth Factor beta1Smad3 ProteinSMAD3 protein, humanTransforming Growth Factor beta1FibrosisHuman umbilical cord-derived mesenchymal stem cellsOvarian stromal cellsPrimary ovarian insufficiencyTGF-β1 signaling pathway

Identifiers

PMID32894203
PMCPMC7487655
OpenAlexW3084431037

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.