ArticleInsect science2021
CRISPR/Cas9-based functional analysis of yellow gene in the diamondback moth, Plutella xylostella.
Article in Insect science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The trial behind it
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Who cites it
11 citing papers in PubMed.
- Yellow protein co-opted to sustain obligate symbiosis in leaf beetles.Nature communications · 2026Article
- Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.eLife · 2026Article
- Harnessing CRISPR-Cas technology for insect pest control: current advances and future perspectives.Molecular biology reports · 2026Review
- Disruption of yellow-e3 impairs both adult molting and cuticular melanization in the honeybee (Apis mellifera).Frontiers in zoology · 2026Article
- Highly Efficient Generation of Germline Mutations Using CRISPR/Cas9 in the Speckled Wood ButterflyEcology and evolution · 2025Article
- CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects.International journal of molecular sciences · 2025Review
- CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Biomolecules · 2024Review
- Allele-specific knockouts reveal a role for apontic-like in the evolutionary loss of larval melanin pigmentation in the domesticated silkworm, Bombyx mori.Insect molecular biology · 2022Article
- Development of CRISPR/Cas9-Mediated Gene-Drive Construct Targeting the Phenotypic Gene inFrontiers in physiology · 2022Article
- Yellow-y Functions in Egg Melanization and Chorion Morphology of the Asian Tiger Mosquito,Frontiers in cell and developmental biology · 2021Article
- Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Protein 9 Mediated Knockout Reveals Functions of theFrontiers in physiology · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The diamondback moth, Plutella xylostella (L.), is an economically important pest of cruciferous crops worldwide. This pest is notorious for rapid evolution of the resistance to different classes of insecticides, making it increasingly difficult to control. Genetics-based control approaches, through manipulation of target genes, have been reported as promising supplements or alternatives to traditional methods of pest management. Here we identified a gene of pigmentation (yellow) in P. xylostella, Pxyellow, which encodes 1674 bp complementary DNA sequence with four exons and three introns. Using the clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR-associated protein 9 system, we knocked out Pxyellow, targeting two sites in Exon III, to generate 272 chimeric mutants (57% of the CRISPR-treated individuals) with color-changed phenotypes of the 1st to 3rd instar larvae, pupae, and adults, indicating that Pxyellow plays an essential role in the body pigmentation of P. xylostella. Fitness analysis revealed no significant difference in the oviposition of adults, the hatchability of eggs, and the weight of pupae between homozygous mutants and wildtypes, suggesting that Pxyellow is not directly involved in regulation of growth, development, or reproduction. This work advances our understanding of the genetic and insect science molecular basis for body pigmentation of P. xylostella, and opens a wide avenue for development of the genetically based pest control techniques using Pxyellow as a screening marker.
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