ArticleLaboratory investigation; a journal of technical methods and pathology2021
Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo.
Article in Laboratory investigation; a journal of technical methods and pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- A photoactivatable Cre-loxP system for spatiotemporal genetic manipulation in mouse taste buds.The Journal of biological chemistry · 2026Article
- A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice.Nucleic acids research · 2025Article
- Optogenetic control of early embryos labeling using photoactivatable Cre recombinase 3.0.FEBS open bio · 2024Article
- Need of orthogonal approaches in neurological disease modeling in mouse.Frontiers in molecular neuroscience · 2024Review
- Perfect duet: Dual recombinases improve genetic resolution.Cell proliferation · 2023Review
- Review
- Stable Transgenic Mouse Strain with Enhanced Photoactivatable Cre Recombinase for Spatiotemporal Genome Manipulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing.Nature communications · 2022Article
- Establishment of a Cre-loxP System Based on a LeakyMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the Cre-loxP recombination system has been extensively used to analyze gene function in vivo, spatiotemporal control of Cre activity is a critical limitation for easy and precise recombination. Here, we established photoactivatable-Cre (PA-Cre) knock-in (KI) mice at a safe harbor locus for the spatial and temporal regulation of Cre recombinase activity. The mice showed whole-body Cre recombination activity following light exposure for only 1 h. Almost no leaks of Cre recombination activity were detected in the KI mice under natural light conditions. Spot irradiation could induce locus-specific recombination noninvasively, enabling us to compare phenotypes on the left and right sides in the same mouse. Furthermore, long-term irradiation using an implanted wireless LED substantially improved Cre recombination activity, especially in the brain. These results demonstrate that PA-Cre KI mice can facilitate the spatiotemporal control of genetic engineering and provide a useful resource to elucidate gene function in vivo with Cre-loxP.
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Registered trials
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