Evidence map›Paper›PMID 32888429›Full record

ArticleCell2020

The Human and Mouse Enteric Nervous System at Single-Cell Resolution.

Eugene Drokhlyansky, Christopher S Smillie, Nicholas Van Wittenberghe, Maria Ericsson, Gabriel K Griffin, Gokcen Eraslan, Danielle Dionne, Michael S Cuoco, Max N Goder-Reiser, Tatyana Sharova and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 371 papers.

0numbers the graph read from it
0cells of the map it votes in
371citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

371 citing papers in PubMed.

  1. RNASEH2B loss and PARP inhibition in advanced prostate cancer.The Journal of clinical investigation · 2024
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  5. Blocking the mCellular & molecular immunology · 2026
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  11. Review
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  13. Article
  14. Article
  15. RareInternational journal of molecular sciences · 2026
    Article
  16. Intestinal muscularis in health and disease.Communications biology · 2026
    Review
  17. Article
  18. Article
  19. Article
  20. Article

311 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Eugene DrokhlyanskyKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Christopher S SmillieKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Nicholas Van WittenbergheKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Maria EricssonDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.
Gabriel K GriffinBroad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Gokcen EraslanKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Danielle DionneKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Michael S CuocoKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Max N Goder-ReiserMassachusetts General Hospital, Cambridge, MA, USA.
Tatyana SharovaMassachusetts General Hospital, Cambridge, MA, USA.
Olena KuksenkoKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Andrew J AguirreBroad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA, USA.
Genevieve M BolandDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA.
Daniel GrahamBroad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA; Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA, USA.
Orit Rozenblatt-RosenKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. Electronic address: orit@broadinstitute.org.
Ramnik J XavierBroad Institute of MIT and Harvard, Cambridge, MA, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA; Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA, USA; Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA. Electronic address: xavier@molbio.mgh.harvard.edu.
Aviv RegevKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA; Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: aregev@broadinstitute.org.

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Ramnik J Xavier · 1991 to 2026
$35.3M
Systematic dissection of gut cellular circuits [at single cell resolution]RC2DK114784 · NIDDK · BROAD INSTITUTE, INC. · PI ASHENBERG, ORR, GRAHAM, DANIEL BARTHOLOMEW · 2017 to 2021
$9.0M
Lysosome Homeostasis and Microbicidal Mechanisms in Innate ImmunityR01DK117263 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI XAVIER, RAMNIK J · 2018 to 2021
$2.4M
Functional interrogation of epigenetic vulnerabilities in KRAS-mutant pancreatic cancerK08CA218420 · NCI · DANA-FARBER CANCER INST · PI AGUIRRE, ANDREW JAMES · 2017 to 2021
$886k
Howard Hughes Medical InstituteNCI NIH HHS K08 CA218420NIDDK NIH HHS P30 DK043351NIDDK NIH HHS R01 DK117263NIDDK NIH HHS RC2 DK114784
6 · The paper itself

Abstract

The enteric nervous system (ENS) coordinates diverse functions in the intestine but has eluded comprehensive molecular characterization because of the rarity and diversity of cells. Here we develop two methods to profile the ENS of adult mice and humans at single-cell resolution: RAISIN RNA-seq for profiling intact nuclei with ribosome-bound mRNA and MIRACL-seq for label-free enrichment of rare cell types by droplet-based profiling. The 1,187,535 nuclei in our mouse atlas include 5,068 neurons from the ileum and colon, revealing extraordinary neuron diversity. We highlight circadian expression changes in enteric neurons, show that disease-related genes are dysregulated with aging, and identify differences between the ileum and proximal/distal colon. In humans, we profile 436,202 nuclei, recovering 1,445 neurons, and identify conserved and species-specific transcriptional programs and putative neuro-epithelial, neuro-stromal, and neuro-immune interactions. The human ENS expresses risk genes for neuropathic, inflammatory, and extra-intestinal diseases, suggesting neuronal contributions to disease.

Indexed as

AgingAnimalsCircadian ClocksColonEndoplasmic Reticulum, RoughEnteric Nervous SystemEpithelial CellsFemaleGene Expression Regulation, DevelopmentalGenetic Predisposition to DiseaseHumansIleumInflammationIntestinal DiseasesMaleMiceRNA, MessengeragingcircadiancolonENSenteric nervous systementeric neuronGWASileumneuro-immunesingle cell

Identifiers

PMID32888429
PMCPMC8358727

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.