Evidence map›Paper›PMID 32888129›Full record

ArticleMolecular biology reports2020

Single-tube detection of nine bacterial antibiotic-resistance genes by a 2-dimensional multiplex qPCR assay based on fluorescence and melting temperature.

Yingxue Li, Ping Xu, Zhenzhou Wan, Hong Du, Xia Jin, Chiyu Zhang

Abstract read
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In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 6 institutions in 1 country.

Yingxue LiShanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Ping XuDepartment of Clinical Laboratory, The Fifth People's Hospital of Suzhou, Suzhou, 215000, China.
Zhenzhou WanMedical Laboratory of Taizhou Fourth People's Hospital, Taizhou, 225300, Jiangsu, China.
Hong DuDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, China. hongdu@suda.edu.cn.
Xia JinShanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Chiyu ZhangShanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China. zhangcy1999@shphc.org.cn.ORCID http://orcid.org/0000-0001-8735-9857
Institut Pasteur of Shanghai · CNShanghai Public Health Clinical Center · CNShanghai University · CNSoochow University · CNTaizhou Fourth People's Hospital · CNThe Fifth People’s Hospital of Suzhou · CN

Funding

Chinese Academy of Sciences 153831KYSB20170043National Major Science and Technology Projects of China 2017ZX10103009-002
6 · The paper itself

Abstract

Simple, multiplex qPCR methods are advantages for rapid molecular diagnosis of multiple antibiotics-resistant genes simultaneously. However, the number of genes can be detected in a single reaction tube is often limited by the fluorescence channels of a real-time PCR instrument. In this study, we developed a simple 2-D multiplex qPCR method by combining the probe colors and amplicon Tm values to overcome the mechanical limit of the machine. The principle of the novel assay was validated by detection of nine bacterial antibiotic-resistance genes (KPC, NDM, VIM, OXA-48, GES, CIT, EBC, ACC and DHA) in a single reaction tube. This assay is highly sensitive within a range of 30-3000 copies per reaction. The simplicity, rapidity, high sensitivity and specificity, and low cost of the novel method make it a promising tool for developing clinical diagnostic kits for monitoring resistance and other genetic determinants of infectious diseases.

Indexed as

Anti-Bacterial AgentsMultiplex Polymerase Chain ReactionBacteriaBacterial ProteinsDrug Resistance, BacterialSensitivity and SpecificityAnti-Bacterial AgentsBacterial Proteins2-D multiplex qPCRAmpliconAntibiotic-resistance geneFluorescence probeMelting curveMelting temperature (Tm)

Identifiers

PMID32888129
OpenAlexW3083365581

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.