ArticlePlant cell reports2021
GhWRKY21 regulates ABA-mediated drought tolerance by fine-tuning the expression of GhHAB in cotton.
Article in Plant cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Genome-Wide Identification and Characterization of the Key Genes for Salicylic Acid Biosynthesis in Four Cotton Species.International journal of molecular sciences · 2026Article
- A FnWRKY17-Frontiers in plant science · 2026Article
- Overexpression ofPlants (Basel, Switzerland) · 2025Article
- A WRKY Transcription Factor,Plants (Basel, Switzerland) · 2025Article
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
- GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton.Computational and structural biotechnology journal · 2024Article
- Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein-Protein Interactions in Cell Signaling Pathways.Biomolecules · 2024Review
- Genome-Wide Identification ofGenes · 2024Article
- TaWRKY31, a novel WRKY transcription factor in wheat, participates in regulation of plant drought stress tolerance.BMC plant biology · 2024Article
- Genome-wide identification and comprehensive analysis of WRKY transcription factor family in safflower during drought stress.Scientific reports · 2023Article
- Integrated metabolic and transcriptional analysis reveals the role of carotenoid cleavage dioxygenase 4 (IbCCD4) in carotenoid accumulation in sweetpotato tuberous roots.Biotechnology for biofuels and bioproducts · 2023Article
- Characterization of theInternational journal of molecular sciences · 2023Article
- Multifaceted roles of WRKY transcription factors in abiotic stress and flavonoid biosynthesis.Frontiers in plant science · 2023Review
- A Comprehensive Gene Co-Expression Network Analysis Reveals a Role ofInternational journal of molecular sciences · 2022Article
- Regulatory network established by transcription factors transmits drought stress signals in plant.Stress biology · 2022Review
- Genome-Wide Identification and Characterization of the WRKY Gene Family inInternational journal of molecular sciences · 2022Article
- Identification of drought responsive Elaeis guineensis WRKY transcription factors with sensitivity to other abiotic stresses and hormone treatments.BMC genomics · 2022Article
- WRKY transcription factors (TFs): Molecular switches to regulate drought, temperature, and salinity stresses in plants.Frontiers in plant science · 2022Review
- Targeted and untargeted metabolomics reveals deep analysis of drought stress responses in needles and roots ofFrontiers in plant science · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
key messageWe report that GhWRKY21, a WRKY transcription factor, plays essential roles in regulating the intensity of the drought-induced ABA signalling pathway by facilitating the expression of GhHAB in cotton (Gossypium hirsutum). Abscisic acid (ABA) is one of the most important plant hormones in response to abiotic stress. However, activation of the ABA signalling pathway often leads to growth inhibition. The mechanisms that regulate the intensity of ABA signals are poorly understood. Here, we isolated and analysed the cotton group IId WRKY transcription factor (TF) gene GhWRKY21. Functional analysis indicated that GhWRKY21 plays a negative role in the drought response of cotton. Silencing of GhWRKY21 in cotton dramatically increased drought tolerance, whereas ectopic GhWRKY21 overexpression in Nicotiana benthamiana decreased drought tolerance. Furthermore, the GhWRKY21-mediated drought tolerance was ABA dependent. To clarify the mechanism underlying the GhWRKY21-mediated regulation of drought tolerance, 17 clade-A-type type 2C protein phosphatase (PP2C) genes, which are negative regulators of ABA signalling, were identified in cotton. Notably, GhWRKY21 interacted specifically with the W-box element within the promoter of GhHAB and regulated its expression. Silencing of GhHAB in cotton yielded a phenotype similar to that of GhWRKY21-silenced cotton. These results suggest that GhWRKY21 regulates the intensity of ABA signals by facilitating the expression of GhHAB. In summary, these findings dramatically improve our understanding of the function of WRKY TFs and provide insights into the mechanism of ABA-mediated drought tolerance.
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