ArticleThe ISME journal2021
The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity.
Article in The ISME journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Designing a Synthetic Microbial Community to Enhance Flavor Compound Production in Sesame Flavor-TypeFoods (Basel, Switzerland) · 2026Article
- Membrane perturbation by the last-resort antibiotic polymyxin B drives biphasic regulation of horizontal gene transfer.The ISME journal · 2026Article
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- Boundary conditions for exploiting the cooperation ofApplied and environmental microbiology · 2025Article
- Resource-use plasticity governs the causal relationship between traits and community structure in model microbial communities.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Construction of an Efficient Engineered Strain for Chaetoglobosin A Bioresource Production from Potato Starch Industrial Waste.Foods (Basel, Switzerland) · 2025Article
- Different bacterial growth of major mastitis pathogens after coculturing withFrontiers in microbiology · 2025Article
- Enhanced Oil Recovery in a Co-Culture System ofMicroorganisms · 2024Article
- Set Up and Utilization of a Three-Dimensional In Vitro Bioreactor System for Human Intestinal Studies and Microbial Co-Cultures.Current protocols · 2024Article
- The repertoire and levels of secondary metabolites in microbial cocultures depend on the inoculation ratio: a case study involving Aspergillus terreus and Streptomyces rimosus.Biotechnology letters · 2024Article
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- Formation of a constructed microbial community in a nutrient-rich environment indicates bacterial interspecific competition.mSystems · 2024Article
- Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium.Nature communications · 2024Article
- Evaluation of ready-to-use freezer stocks of a synthetic microbial community for maize root colonization.Microbiology spectrum · 2024Article
- Strategies for biofilm optimization of plastic-degrading microorganisms and isolating biofilm formers from plastic-contaminated environments.Sustainable microbiology · 2024Article
- Metabolic interactions affect the biomass of synthetic bacterial biofilm communities.mSystems · 2023Article
- Control points for design of taxonomic composition in synthetic human gut communities.Cell systems · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture system with two model bacteria in various carbon sources and investigated the influence of initial inoculum ratios of 1:1000 to 1000:1 on community structure, function, and bacterial interaction. We found that the final ratio of the cocultures with different initial inoculum ratios differed in approximately five-sixths of the carbon sources, suggesting that the final ratio is highly dependent on the initial inoculum ratio, while the carbon source preferences of bacteria could not predict the final ratio of cocultures. Furthermore, we found that the initial ratio could regulate the metabolic capacity of the coculture, as only cocultures with initial ratios of 1:1 and 1000:1 gained high capacity on 14 specific carbon sources. The underlying reason may be that the pattern of species interaction is changed by the initial ratio. In conclusion, we showed that the initial ratio can induce emergent properties in coculture. These findings suggest that the initial ratio not only impacts the reproducibility of coculture experiments but also can influence our understanding of generic microbial ecology.
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