ArticleScientific reports2020
Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 18 citations in OpenAlex.
- Cytokine-induced chromatin accessibility in whole blood neutrophils links to sepsis transcriptional states.Frontiers in immunology · 2026Article
- Mechanical force-induced oncostatin M secretion by Jun-positive neutrophils promotes craniofacial bone regeneration for midface hypoplasia treatment.Stem cell research & therapy · 2025Article
- A New Easy-to-Perform Flow Cytometry Assay for Determining Bacterial- and Viral-Infection-Induced Polymorphonuclear Neutrophil and Monocyte Membrane Marker Modulation in Febrile Patients.International journal of molecular sciences · 2024Article
- Human Neutrophil Elastase: Characterization of Intra- vs. Extracellular Inhibition.International journal of molecular sciences · 2024Article
- The effect of whole blood logistics on neutrophil non-specific activation and kinetics ex vivo.Scientific reports · 2024Article
- Article
- Isolation methods determine human neutrophil responses after stimulation.Frontiers in immunology · 2023Article
- Lipocalin-2 and neutrophil activation in pancreatic cancer cachexia.Frontiers in immunology · 2023Article
- Neutrophil activation and circulating neutrophil extracellular traps are increased in venous thromboembolism patients for at least one year after the clinical event.Journal of thrombosis and thrombolysis · 2022Article
- Method Matters: Effect of Purification Technology on Neutrophil Phenotype and Function.Frontiers in immunology · 2022Article
- Article
- Bacterial phagocytosis and reactive oxygen species production by camel neutrophils and monocytes are influenced by the type of anticoagulation agent.Veterinary world · 2021Article
- Is Elevated Neutrophil Count and Neutrophil-to-Lymphocyte Ratio a Cause or Consequence of Schizophrenia?-A Scoping Review.Frontiers in psychiatry · 2021Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the isolation of polymorphonuclear neutrophils (PMNs) the technique and other external factors can have great influence on the quality and quantity of isolated neutrophils. To elucidate the influence of the blood collection technique, anticoagulants and storing temperature on isolated PMNs healthy volunteers provided blood samples with different needles and collection techniques, anticoagulants (EDTA, heparin, citrate) and storing temperatures (4, 22, 37 °C). From each blood sample PMNs were isolated and compared regarding number of PMNs and oxidative burst. The blood collection technique, anticoagulants and storing temperature had minor impact on isolated PMNs. All three tested cannulas and anticoagulants can be used to obtain blood samples for PMN isolation. For storing temperatures 37 °C should be preferred. Regarding time between the PMN isolation and the actual experiments, a time span of maximum 1 h should be targeted.
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