ArticleScientific reports2020
Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 78 citations in OpenAlex.
- Global Proteomic Analysis of Sperm and Seminal Plasma From Tropically-Adapted Rams With Contrasting Parameters of Frozen-Thawed Semen.Molecular reproduction and development · 2026Article
- The maternal immune response to semen: implications for reproductive success in livestock.Reproduction & fertility · 2026Review
- SemProtDB: A Reference Database of Seminal Plasma Proteins.Molecular reproduction and development · 2026Article
- Effects of Selenium Nanoparticles and Sodium Selenite Supplementation on Cryopreserved Ram Sperm Quality, Oxidative Status, and PRDX5 Gene Expression.Animals : an open access journal from MDPI · 2026Article
- Proteome of seminal plasma and sperm associated with sperm survival following cryopreservation in the Red Wolf (Canis rufus).Scientific reports · 2025Article
- High-throughput proteomic profiling reveals dysregulated serum proteins in low fertility bulls linked to nutrient-autophagy-inflammation-extracellular matrix axis.Veterinary research communications · 2025Article
- Sperm protein profiles and their correlation with DNA integrity and protamine deficiency in Donggala bullsVeterinary world · 2025Article
- Integrated Proteomics and Metabolomics Reveal Spermine Enhances Sperm Freezability via Antioxidant Pathways.Antioxidants (Basel, Switzerland) · 2025Article
- Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.Journal of proteome research · 2025Article
- Decoding bull fertility in vitro: a proteomics exploration from sperm to blastocyst.Reproduction (Cambridge, England) · 2025Article
- A Broad Genome Survey Reveals Widespread Presence of Secretoglobin Genes in Squamate and Archosaur Reptiles that Flowered into Diversity in Mammals.Genome biology and evolution · 2025Article
- Genomic evidence of improved fertility and adaptation in Iranian domestic sheep attributed to introgression from Asiatic Mouflon and urial.Scientific reports · 2025Article
- Seminal Plasma Proteomics Using Filter-Aided Sample Preparation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Proteostasis as a Sentry for Sperm Quality and Male Fertility.Advances in experimental medicine and biology · 2025Review
- Proteomic analysis of Toraya buffalo seminal plasma and sperm: uncovering insights to optimize reproductive success.Frontiers in veterinary science · 2025Article
- Extender development for optimal cryopreservation of buck sperm to increase reproductive efficiency of goats.Frontiers in veterinary science · 2025Review
- Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics.Veterinary world · 2025Article
- Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls.Veterinary world · 2025Article
- Effect of Thawing Procedure and Thermo-Resistance Test on Sperm Motility and Kinematics Patterns in Two Bovine Breeds.Animals : an open access journal from MDPI · 2024Article
- Seminal cell-free DNA as a potential marker for in vitro fertility of Nellore bulls.Journal of assisted reproduction and genetics · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
The present study investigated the seminal plasma proteome of Holstein bulls with low (LF; n = 6) and high (HF; n = 8) sperm freezability. The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cytometry varied from -2.2 in LF to + 7.8 in HF bulls, as compared to the average %ViableSperm (54.7%) measured in an 860-sire population. Seminal proteins were analyzed by label free mass spectrometry, with the support of statistical and bioinformatics analyses. This approach identified 1,445 proteins, associated with protein folding, cell-cell adhesion, NADH dehydrogenase activity, ATP-binding, proteasome complex, among other processes. There were 338 seminal proteins differentially expressed (p < 0.05) in LF and HF bulls. Based on multivariate analysis, BSP5 and seminal ribonuclease defined the HF phenotype, while spermadhesin-1, gelsolin, tubulins, glyceraldehyde-3-phosphate dehydrogenase, calmodulin, ATP synthase, sperm equatorial segment protein 1, peroxiredoxin-5, secretoglobin family 1D and glucose-6-phosphate isomerase characterized the LF phenotype. Regression models indicated that %ViableSperm of bulls was related to seminal plasma peroxiredoxin-5, spermadhesin-1 and the spermadhesin-1 × BSP5 interaction (R
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.