ArticleBMC biology2020
CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA.
Article in BMC biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- RNA Tailing by Nucleotidyltransferases in Plants: Mechanisms, Functions, and Biological Significance.Plants (Basel, Switzerland) · 2026Review
- Poly(U) polymerase activity promotes establishment of primordial germ cells and limits somatic PGL granules in the Caenorhabditis elegans embryo.Developmental biology · 2026Article
- The ribonuclease DISL-2 mediates piRNA degradation in C. elegans.Cell reports · 2025Article
- A targeted genetic screen identifies Caenorhabditis elegans genes involved in RNAi-independent antiviral defense.Virology · 2025Article
- Distinct regulatory mechanisms by the nuclear Argonautes HRDE-1 and NRDE-3 in the soma of Caenorhabditis elegans.G3 (Bethesda, Md.) · 2025Article
- Two eIF4E paralogs occupy separate germ granule messenger ribonucleoproteins that mediate mRNA repression and translational activation.Genetics · 2025Article
- Poly(U) polymerase activity in Caenorhabditis elegans regulates abundance and tailing of sRNA and mRNA.Genetics · 2024Article
- Collagen and actin network mediate antiviral immunity against Orsay virus in C. elegans intestinal cells.PLoS pathogens · 2024Article
- Article
- Target-dependent suppression of siRNA production modulates the levels of endogenous siRNAs in the Caenorhabditis elegans germline.Development (Cambridge, England) · 2022Article
- Antisense ribosomal siRNAs inhibit RNA polymerase I-directed transcription in C. elegans.Nucleic acids research · 2021Article
- Non-Coding RNA-Driven Regulation of rRNA Biogenesis.International journal of molecular sciences · 2020Review
- CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA.BMC biology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundModification of RNAs, particularly at the terminals, is critical for various essential cell processes; for example, uridylation is implicated in tumorigenesis, proliferation, stem cell maintenance, and immune defense against viruses and retrotransposons. Ribosomal RNAs can be regulated by antisense ribosomal siRNAs (risiRNAs), which downregulate pre-rRNAs through the nuclear RNAi pathway in Caenorhabditis elegans. However, the biogenesis and regulation of risiRNAs remain obscure. Previously, we showed that 26S rRNAs are uridylated at the 3'-ends by an unknown terminal polyuridylation polymerase before the rRNAs are degraded by a 3' to 5' exoribonuclease SUSI-1(ceDIS3L2).
resultsHere, we found that CDE-1, one of the three C.elegans polyuridylation polymerases (PUPs), is specifically involved in suppressing risiRNA production. CDE-1 localizes to perinuclear granules in the germline and uridylates Argonaute-associated 22G-RNAs, 26S, and 5.8S rRNAs at the 3'-ends. Immunoprecipitation followed by mass spectrometry (IP-MS) revealed that CDE-1 interacts with SUSI-1(ceDIS3L2). Consistent with these results, both CDE-1 and SUSI-1(ceDIS3L2) are required for the inheritance of RNAi.
conclusionsThis work identified a rRNA surveillance machinery of rRNAs that couples terminal polyuridylation and degradation.
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