Evidence map›Paper›PMID 32887585›Full record

ArticleJournal of biomedical science2020

Copy number variant hotspots in Han Taiwanese population induced pluripotent stem cell lines - lessons from establishing the Taiwan human disease iPSC Consortium Bank.

Ching-Ying Huang, Ling-Hui Li, Wan-Tseng Hsu, Yu-Che Cheng, Martin W Nicholson, Chun-Lin Liu, Chien-Yu Ting, Hui-Wen Ko, Shih-Han Syu, Cheng-Hao Wen and 15 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
  2. Review
  3. Replication stress causes delayed mitotic entry and chromosome 12 fragility at the ANKS1B large neuronal gene in human induced pluripotent stem cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023
    Article
  4. Article
  5. Review
  6. Article
  7. Cardio- and Neurotoxicity of Selected Anti-COVID-19 Drugs.Pharmaceuticals (Basel, Switzerland) · 2022
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Ching-Ying HuangInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Ling-Hui LiInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Wan-Tseng HsuSchool of Pharmacy, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Yu-Che ChengInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Martin W NicholsonInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Chun-Lin LiuInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Chien-Yu TingInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Hui-Wen KoBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan.
Shih-Han SyuBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan.
Cheng-Hao WenBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan.
Zhuge YanStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Hsiang-Po HuangGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Hong-Lin SuDepartment of Life Sciences, National Chung-Hsing University, Taichung, 402, Taiwan.
Po-Min ChiangInstitute of Clinical Medicine, National Cheng Kung University, Tainan, 701, Taiwan.
Chia-Ning ShenGenomics Research Center, Academia Sinica, Taipei, 115, Taiwan.
Hsin-Fu ChenGraduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
B Lin Ju YenInstitute of Cellular and System Medicine, National Health Research Institutes, Zhunan, 350, Taiwan.
Huai-En LuBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan.
Shiaw-Min HwangBioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, 300, Taiwan.
Shih-Hwa ChiouInstitute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, 112, Taiwan.
Hong-Nerng HoDepartment of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei, Taiwan.
Jer-Yuarn WuInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.
Timothy J KampStem Cell and Regenerative Medicine Center, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Joseph C WuStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Patrick C H HsiehInstitute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan. phsieh@ibms.sinica.edu.tw.ORCID http://orcid.org/0000-0002-8910-3596

Funding

Ministry of Science and Technology, Taiwan 107-2319-B-001-003Ministry of Science and Technology, Taiwan 107-2321-B-001-029Ministry of Science and Technology, Taiwan 108-2319-B-001-004Ministry of Science and Technology, Taiwan 108-2321-B-001-017Ministry of Science and Technology, Taiwan MOST 109-2740-B-001-002
6 · The paper itself

Abstract

backgroundThe Taiwan Human Disease iPSC Service Consortium was established to accelerate Taiwan's growing stem cell research initiatives and provide a platform for researchers interested in utilizing induced pluripotent stem cell (iPSC) technology. The consortium has generated and characterized 83 iPSC lines: 11 normal and 72 disease iPSC lines covering 21 different diseases, several of which are of high incidence in Taiwan. Whether there are any reprogramming-induced recurrent copy number variant (CNV) hotspots in iPSCs is still largely unknown.

methodsWe performed genome-wide copy number variant screening of 83 Han Taiwanese iPSC lines and compared them with 1093 control subjects using an Affymetrix genome-wide human SNP array.

resultsIn the iPSCs, we identified ten specific CNV loci and seven "polymorphic" CNV regions that are associated with the reprogramming process. Additionally, we established several differentiation protocols for our iPSC lines. We demonstrated that our iPSC-derived cardiomyocytes respond to pharmacological agents and were successfully engrafted into the mouse myocardium demonstrating their potential application in cell therapy.

conclusionsThe CNV hotspots induced by cell reprogramming have successfully been identified in the current study. This finding may be used as a reference index for evaluating iPSC quality for future clinical applications. Our aim was to establish a national iPSC resource center generating iPSCs, made available to researchers, to benefit the stem cell community in Taiwan and throughout the world.

Indexed as

Cell DifferentiationDNA Copy Number VariationsAdolescentAdultAgedAged, 80 and overCellular ReprogrammingChildChild, PreschoolFemaleHumansInduced Pluripotent Stem CellsMaleMiddle AgedMyocytes, CardiacTaiwanCell differentiationCopy number variantDrug screeningHotspotHuman induced pluripotent stem cellStem cell bank

Identifiers

PMID32887585
PMCPMC7487458

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.