ArticlePloS one2020
Corneal dysfunction precedes the onset of hyperglycemia in a mouse model of diet-induced obesity.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Platelet extravasation in the microvasculature: an under-appreciated role for platelets.Frontiers in immunology · 2026Review
- Obesity negatively impacts corneal nerves in patients with diabetes mellitus.Eye and vision (London, England) · 2025Article
- Evaluation of the Effect of Body Mass Index and Waist Circumference on Ocular Health Parameters in Children and Adolescents.Children (Basel, Switzerland) · 2025Article
- Impact of Obesity and Age on Mouse Corneal Innervation at the Epithelial-Stromal Interface.Investigative ophthalmology & visual science · 2024Article
- Review
- Review
- In vivo corneal confocal microscopy as a non-invasive test to assess obesity induced small fibre nerve damage and inflammation.Eye (London, England) · 2023Observational
- A Low-Fat/Sucrose Diet Rich in Complex Carbohydrates Reverses High-Fat/Sucrose Diet-Induced Corneal Dysregulation.International journal of molecular sciences · 2023Article
- Association of Serum Lipid Level with Meibum Biosynthesis and Meibomian Gland Dysfunction: A Review.Journal of clinical medicine · 2022Review
- High-Fat Nutritional Challenge Reshapes Circadian Signatures in Murine Extraorbital Lacrimal Glands.Investigative ophthalmology & visual science · 2022Article
- S1P promotes corneal trigeminal neuron differentiation and corneal nerve repair via upregulating nerve growth factor expression in a mouse model.Open life sciences · 2022Article
- High fat diet induced gut dysbiosis alters corneal epithelial injury response in mice.The ocular surface · 2022Article
- An Epithelial Abrasion Model for Studying Corneal Wound Healing.Journal of visualized experiments : JoVE · 2021Article
- Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet.Nutrients · 2021Article
- Platelet and Erythrocyte Extravasation across Inflamed Corneal Venules Depend on CD18, Neutrophils, and Mast Cell Degranulation.International journal of molecular sciences · 2021Article
- Obese Mice with Dyslipidemia Exhibit Meibomian Gland Hypertrophy and Alterations in Meibum Composition and Aqueous Tear Production.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe purpose of this study was to use a mouse model of diet-induced obesity to determine if corneal dysfunction begins prior to the onset of sustained hyperglycemia and if the dysfunction is ameliorated by diet reversal.
methodsSix-week-old male C57BL/6 mice were fed a high fat diet (HFD) or a normal diet (ND) for 5-15 weeks. Diet reversal (DiR) mice were fed a HFD for 5 weeks, followed by a ND for 5 or 10 weeks. Corneal sensitivity was determined using aesthesiometry. Corneal cytokine expression was analyzed using a 32-plex Luminex assay. Excised corneas were prepared for immunofluorescence microscopy to evaluate diet-induced changes and wound healing. For wounding studies, mice were fed a HFD or a ND for 10 days prior to receiving a central 2mm corneal abrasion.
resultsAfter 10 days of HFD consumption, corneal sensitivity declined. By 10 weeks, expression of corneal inflammatory mediators increased and nerve density declined. While diet reversal restored nerve density and sensitivity, the corneas remained in a heightened inflammatory state. After 10 days on the HFD, corneal circadian rhythms (limbal neutrophil accumulation, epithelial cell division and Rev-erbα expression) were blunted. Similarly, leukocyte recruitment after wounding was dysregulated and accompanied by delays in wound closure and nerve recovery.
conclusionIn the mouse, obesogenic diet consumption results in corneal dysfunction that precedes the onset of sustained hyperglycemia. Diet reversal only partially ameliorated this dysfunction, suggesting a HFD diet may have a lasting negative impact on corneal health that is resistant to dietary therapeutic intervention.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.