ArticleJournal of assisted reproduction and genetics2020
Evidence of metabolic activity during low-temperature ovarian tissue preservation in different media.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Label-free viability assessment ofBiomedical optics express · 2026Article
- Incorporation of lipids improves cryo-tolerance of vitrified gorgonian coral oocytes.PloS one · 2026Article
- Real-Time Viability Assessment of Ex Vivo Mouse Kidneys for Transplant Applications Using Dynamic Optical Coherence Tomography.bioRxiv : the preprint server for biology · 2025Article
- Analysis of Fertility Preservation by Ovarian Tissue Cryopreservation in Pediatric Children in China.Frontiers in endocrinology · 2022Article
- Histidine buffered media maintains pH stabile during cooled transportation of human ovarian tissue.Journal of ovarian research · 2021Article
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Authors and funding
7 authors.
Funding
Abstract
purposeAlthough ovarian tissue transportation has been validated for up to 24 h, there is no standard protocol to date. We aimed to elucidate how existing media currently used for ovarian tissue transportation affect ovarian tissue metabolism and cell viability.
methodsCow ovarian fragments were immersed in 0.9% NaCl solution, IVF medium, Leibovitz 15 medium (L-15), or PBS for 1, 4, or 24 h at 4 °C. Media were analyzed for pH, lactate dehydrogenase (LDH) activity, and glucose, pyruvate, and lactate concentrations, while apoptosis was assessed by TUNEL assays in fixed fragments. Viability rates were assessed by flow cytometry (FACS).
resultsThere were lower pH levels in NaCl at all time points compared with other media. LDH activity increased with time and was lowest in NaCl at 1 and 4 h. There was no significant difference in glucose levels, but a significant pyruvate decrease in L-15 and a significant lactate increase in all media. TUNEL showed apoptosis rates ranging from 0 to 5%. FACS showed a mean of 4% necrotic cells and 15-19% apoptotic cells after 1 h of incubation, but less than 1% necrotic cells and 2-6% apoptotic cells after 24 h in all media.
conclusionOur results indicate marked metabolic activity in ovarian tissue at 4 °C and suggest that cells use internal sources of energy, which may influence transplantation outcomes. This highlights the importance of better understanding whole tissue dynamics to develop a standard protocol for ovarian tissue transportation. Graphical abstract.
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