ArticleEcology and evolution2020
DNA degradation in fish: Practical solutions and guidelines to improve DNA preservation for genomic research.
Article in Ecology and evolution, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- The Complete Chromosome-Level Genome and Mitogenome Assembly ofEcology and evolution · 2026Article
- Molecular detection of feline viruses in free-ranging European wildcats (Felis s. silvestris) of the Harz Mountains, Germany.Archives of virology · 2026Article
- Draft hybrid genome assembly of the photosynthetic sacoglossan Elysia crispata provides insights into sacoglossan biology.BMC genomics · 2026Article
- Bacterial Community Composition and Functional Potential of the Kleptoplastic Sea SlugBiomolecules · 2026Article
- De novo whole genome assembly of the globally invasive green shore crab Carcinus maenas (Linnaeus, 1758) via long-read Oxford Nanopore MinION sequencing.The Journal of heredity · 2026Article
- Mitogenome Characteristics and Phylogenetic Analysis of SixAnimals : an open access journal from MDPI · 2026Article
- A framework for optimising arthropod DNA quality and quantity for modern sequencing tools using hard ticks (Ixodidae).Parasitology research · 2026Article
- Habitat Specialisation Impacts Clownfish Demographic Resilience to Pleistocene Sea-Level Fluctuations.Molecular ecology · 2025Article
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- Perish the thawed? EDTA reduces DNA degradation during extraction from frozen tissue.PloS one · 2025Article
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- Effect of Soybean Protein Concentrate Preparation on Copy Numbers and Structural Characteristics of DNA from Genetically Modified Soybean.Foods (Basel, Switzerland) · 2023Article
- Draft genome assemblies of the avian louse Brueelia nebulosa and its associates using long-read sequencing from an individual specimen.G3 (Bethesda, Md.) · 2023Article
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- Greater than pH 8: The pH dependence of EDTA as a preservative of high molecular weight DNA in biological samples.PloS one · 2023Article
- Article
- Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing.GigaScience · 2022Article
- Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators.Frontiers in genetics · 2021Article
- DNA degradation in fish: Practical solutions and guidelines to improve DNA preservation for genomic research.Ecology and evolution · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The more demanding requirements of DNA preservation for genomic research can be difficult to meet when field conditions limit the methodological approaches that can be used or cause samples to be stored in suboptimal conditions. Such limitations may increase rates of DNA degradation, potentially rendering samples unusable for applications such as genome-wide sequencing. Nonetheless, little is known about the impact of suboptimal sampling conditions. We evaluated the performance of two widely used preservation solutions (1. DESS: 20% DMSO, 0.25 M EDTA, NaCl saturated solution, and 2. Ethanol >99.5%) under a range of storage conditions over a three-month period (sampling at 1 day, 1 week, 2 weeks, 1 month, and 3 months) to provide practical guidelines for DNA preservation. DNA degradation was quantified as the reduction in average DNA fragment size over time (DNA fragmentation) because the size distribution of DNA segments plays a key role in generating genomic datasets. Tissues were collected from a marine teleost species, the Australasian snapper,
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.