ArticlePlant methods2020
Efficient chromatin profiling of H3K4me3 modification in cotton using CUT&Tag.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed.
- Bivalent chromatin states and epigenetic priming in cotton: the role of H3K4me3 and H3K27me3 in cold stress memory and resilience.Epigenetics & chromatin · 2026Review
- Dynamics of H3K4me3 and H3K36me3 histone modifications in response to powdery mildew infection in common wheat.BMC plant biology · 2026Article
- ZmSKIP enhances drought tolerance by reducing stomatal aperture in maize.PLoS genetics · 2026Article
- CUT&Tag for High-Resolution Epigenomic Profiling From a Low Amount ofPlant direct · 2026Article
- CUT&tag analysis reveals a crucial role of H3K4 trimethylation occupancy in cold-related genes by cold stress on cotton.BMC plant biology · 2026Article
- Many roads lead to a plant cistrome: mapping and interpreting transcription factor binding in plants.Genome biology · 2026Review
- Molecular networks and signaling pathways governing abiotic stress tolerance in cotton: advances and perspectives.Functional & integrative genomics · 2026Review
- Lactylation at H3K18 drives pathological angiogenesis via metabolic-epigenetic crosstalk in ischemic retinopathy.Theranostics · 2026Article
- Cleavage Under Targets and Tagmentation (CUT&Tag) Protocol for Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- RELA Ablation Contributes to Progression of Hepatocellular Carcinoma with TP53Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The GoSPGF-GoPGF-GhWRKYs transcriptional cascade positively regulates pigment gland formation in the stem of upland cotton.Plant biotechnology journal · 2025Article
- The Association Between DNA Methylation and Three-Dimensional Genome During Whole Genome Doubling inPlants (Basel, Switzerland) · 2025Article
- Intra species dissection of phytophthora capsici resistance in black pepper.Journal of advanced research · 2025Article
- Exploring the untapped potential of single-cell and spatial omics in plant biology.The New phytologist · 2025Review
- Redox-Epigenetic Crosstalk in Plant Stress Responses: The Roles of Reactive Oxygen and Nitrogen Species in Modulating Chromatin Dynamics.International journal of molecular sciences · 2025Review
- GhLPF1 Associated Network Is Involved with Cotton Lint Percentage Regulation Revealed by the Integrative Analysis of Spatial Transcriptome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- TTLOC: A Tn5 transposase-based approach to localize T-DNA integration sites.Plant physiology · 2025Article
- ZmHSFA2B self-regulatory loop is critical for heat tolerance in maize.Plant biotechnology journal · 2025Article
- From chromatin to crop: epigenetic innovations in bioenergy systems.Frontiers in plant science · 2025Review
- ZmICE1a regulates the defence-storage trade-off in maize endosperm.Nature plants · 2024Article
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4 authors.
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Abstract
backgroundIn 2019, Kaya-Okur et al. reported on the cleavage under targets and tagmentation (CUT&Tag) technology for efficient profiling of epigenetically modified DNA fragments. It was used mainly for cultured cell lines and was especially effective for small samples and single cells. This strategy generated high-resolution and low-background-noise chromatin profiling data for epigenomic analysis. CUT&Tag is well suited to be used in plant cells, especially in tissues from which small samples are taken, such as ovules, anthers, and fibers.
resultsHere, we present a CUT&Tag protocol step by step using plant nuclei. In this protocol, we quantified the nuclei that can be used in each CUT&Tag reaction, and compared the efficiency of CUT&Tag with chromatin immunoprecipitation with sequencing (ChIP-seq) in the leaves of cotton. A general workflow for the bioinformatic analysis of CUT&Tag is also provided. Results indicated that, compared with ChIP-seq, the CUT&Tag procedure was faster and showed a higher-resolution, lower-background signal than did ChIP.
conclusionA CUT&Tag protocol has been refined for plant cells using intact nuclei that have been isolated.
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