ArticleCell research2020
Structural mechanism of phospholipids translocation by MlaFEDB complex.
Article in Cell research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
- Interactions of outer membrane lipoproteins Pseudomonas aeruginosa PA3214 and Escherichia coli PqiC with their MCE protein binding partners, PA3213 and PqiB.The Journal of biological chemistry · 2026Article
- Mechanistic basis of LolDF-mediated lipoprotein transport in Acinetobacter.Nature communications · 2026Article
- Conformational hijacking of lipoprotein transporter LolDF enables precision antimicrobial activity against Acinetobacter baumannii.Nature communications · 2026Article
- Article
- Following phospholipid transfer through the OmpFProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unveiling multiple copies of MlaC highlights its multifaceted nature.Archives of microbiology · 2025Article
- RamA upregulates the ATP-binding cassette transporterMicrobiology spectrum · 2025Article
- The beneficial health effects of puerarin in the treatment of cardiovascular diseases: from mechanisms to therapeutics.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Structure of the MlaC-MlaD complex reveals molecular basis of periplasmic phospholipid transport.Nature communications · 2024Article
- Maintenance of bacterial outer membrane lipid asymmetry: insight into MlaA.BMC microbiology · 2024Review
- Genetic synergy betweenmBio · 2024Article
- An octameric PqiC toroid stabilises the outer-membrane interaction of the PqiABC transport system.EMBO reports · 2024Article
- Molecular mechanism of phospholipid transport at the bacterial outer membrane interface.Nature communications · 2023Article
- Phospholipid transport to the bacterial outer membrane through an envelope-spanning bridge.bioRxiv : the preprint server for biology · 2023Article
- Article
- Forward or backward, that is the question: phospholipid trafficking by the Mla system.Emerging topics in life sciences · 2023Review
- ABC Transporters in Bacterial Nanomachineries.International journal of molecular sciences · 2023Review
- Structure of an endogenous mycobacterial MCE lipid transporter.Research square · 2023Article
- CHARMM-GUI Enhanced Sampler for various collective variables and enhanced sampling methods.Protein science : a publication of the Protein Society · 2022Article
- Lipid Transport Across Bacterial Membranes.Annual review of cell and developmental biology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Gram-negative bacteria, phospholipids are major components of the inner membrane and the inner leaflet of the outer membrane, playing an essential role in forming the unique dual-membrane barrier to exclude the entry of most antibiotics. Understanding the mechanisms of phospholipid translocation between the inner and outer membrane represents one of the major challenges surrounding bacterial phospholipid homeostasis. The conserved MlaFEDB complex in the inner membrane functions as an ABC transporter to drive the translocation of phospholipids between the inner membrane and the periplasmic protein MlaC. However, the mechanism of phospholipid translocation remains elusive. Here we determined three cryo-EM structures of MlaFEDB from Escherichia coli in its nucleotide-free and ATP-bound conformations, and performed extensive functional studies to verify and extend our findings from structural analyses. Our work reveals unique structural features of the entire MlaFEDB complex, six well-resolved phospholipids in three distinct cavities, and large-scale conformational changes upon ATP binding. Together, these findings define the cycle of structural rearrangement of MlaFEDB in action, and suggest that MlaFEDB uses an extrusion mechanism to extract and release phospholipids through the central translocation cavity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.