ArticleCell research2021
Dissecting the epigenomic dynamics of human fetal germ cell development at single-cell resolution.
Article in Cell research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- A systematic benchmarking framework and dual-view optimization strategy for single-cell DNA methylation imputation.Briefings in bioinformatics · 2026Article
- The road to restore male fertility using in vitro-derived germ cells.Human reproduction update · 2026Review
- A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers.EMBO reports · 2025Article
- Epigenetic regulation by DNA methylation, histone modifications and chromatin remodeling complexes in controlling spermatogenesis and their dysfunction with male infertility.Cellular and molecular life sciences : CMLS · 2025Review
- Single-cell long-read Hi-C, scNanoHi-C2, details 3D genome reorganization in embryonic-stage germ cells.Nature structural & molecular biology · 2025Article
- A survey of human cancer-germline genes: Linking X chromosome localization, DNA methylation and sex-biased expression in early embryos.PLoS genetics · 2025Article
- Epigenetic reprogramming in mouse and human primordial germ cells.Experimental & molecular medicine · 2024Review
- Article
- SMARTdb: An Integrated Database for Exploring Single-cell Multi-omics Data of Reproductive Medicine.Genomics, proteomics & bioinformatics · 2024Article
- Cell-cell communication: new insights and clinical implications.Signal transduction and targeted therapy · 2024Review
- BAllC and BAllCools: efficient formatting and operating for single-cell DNA methylation data.Bioinformatics (Oxford, England) · 2024Article
- The gonadal niche safeguards human fetal germline cell development following maternal SARS-CoV-2 infection.Cell reports. Medicine · 2024Article
- BAllC and BAllCools: Efficient Formatting and Operating for Single-Cell DNA Methylation Data.bioRxiv : the preprint server for biology · 2024Article
- EmptyDropsMultiome discriminates real cells from background in single-cell multiomics assays.Genome biology · 2024Article
- Article
- Dissecting mammalian reproduction with spatial transcriptomics.Human reproduction update · 2023Review
- Reproductive genomics of the mouse: implications for human fertility and infertility.Development (Cambridge, England) · 2023Article
- Resetting histone modifications during human prenatal germline development.Cell discovery · 2023Article
- Epigenetic resetting in the human germ line entails histone modification remodeling.Science advances · 2023Article
- Technologies and applications of single-cell DNA methylation sequencing.Theranostics · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proper development of fetal germ cells (FGCs) is vital for the precise transmission of genetic and epigenetic information through generations. The transcriptional landscapes of human FGC development have been revealed; however, the epigenetic reprogramming process of FGCs remains elusive. Here, we profiled the genome-wide DNA methylation and chromatin accessibility of human FGCs at different phases as well as gonadal niche cells at single-cell resolution. First, we found that DNA methylation levels of FGCs changed in a temporal manner, whereas FGCs at different phases in the same embryo exhibited comparable DNA methylation levels and patterns. Second, we revealed the phase-specific chromatin accessibility signatures at the promoter regions of a large set of critical transcription factors and signaling pathway genes. We also identified potential distal regulatory elements including enhancers in FGCs. Third, compared with other hominid-specific retrotransposons, SVA_D might have a broad spectrum of binding capacity for transcription factors, including SOX15 and SOX17. Finally, using an in vitro culture system of human FGCs, we showed that the BMP signaling pathway promoted the cell proliferation of FGCs, and regulated the WNT signaling pathway by orchestrating the chromatin accessibility of its ligand genes. Our single-cell epigenomic atlas and functional assays provide valuable insights for understanding the strongly heterogeneous, unsynchronized, yet highly robust nature of human germ cell development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.