ArticleScientific reports2020
Deciphering the secretome of leukocyte-platelet rich fibrin: towards a better understanding of its wound healing properties.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Platelet-rich fibrin-derived extracellular vesicles: emerging biological mediators in periodontal regeneration.Odontology · 2026Review
- Refrigerated storage of L-PRF membranes: anti-bacterial activity, cytokine release, andFrontiers in oral health · 2026Article
- The effect of liquid platelet-rich fibrin on oral cells and tissue engineered oral mucosa models in vitro.Scientific reports · 2025Article
- The proteome of circulating extracellular vesicles and their functional effect on platelets vary with the isolation method.Scientific reports · 2025Article
- How to explain the beneficial effects of leukocyte- and platelet-rich fibrin.Periodontology 2000 · 2025Review
- RNAseq of peripheral blood mononucleated cells exposed to platelet-rich fibrin and enamel matrix derivatives.Scientific reports · 2025Article
- Beyond the clot: how the biomolecular landscape of platelet-rich fibrin directs fibroblast functions.Frontiers in oral health · 2025Review
- First- vs. Second-Generation Autologous Platelet Concentrates and Their Implications for Wound Healing: Differences in Proteome and Secretome.Bioengineering (Basel, Switzerland) · 2024Article
- Leukocyte-and Platelet-Rich Fibrin for enhanced tissue repair: an in vitro study characterizing cellular composition, growth factor kinetics and transcriptomic insights.Molecular biology reports · 2024Article
- Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate.Frontiers in bioengineering and biotechnology · 2024Article
- Proteomic Analysis of Mesenchymal Stromal Cells Secretome in Comparison to Leukocyte- and Platelet-Rich Fibrin.International journal of molecular sciences · 2023Article
- Characterization of the Protein Corona of Three Chairside Hemoderivatives on Melt Electrowritten Polycaprolactone Scaffolds.International journal of molecular sciences · 2023Article
- Lipids of Platelet-Rich Fibrin Reduce the Inflammatory Response in Mesenchymal Cells and Macrophages.Cells · 2023Article
- Pure Platelet and Leukocyte-Platelet-Rich Plasma for Regenerative Medicine in Orthopedics-Time- and Preparation-Dependent Release of Growth Factors and Effects on Synovial Fibroblasts: A Comparative Analysis.International journal of molecular sciences · 2023Article
- Blood-derived biomaterials for tissue graft biofabrication by solvent-based extrusion bioprinting.International journal of bioprinting · 2023Review
- Emerging treatment strategies in wound care.International wound journal · 2022Review
- Quality Analysis of Autologous Platelet-Rich Plasmapheresis.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2022Article
- Role of fibroblasts in wound healing and tissue remodeling on Earth and in space.Frontiers in bioengineering and biotechnology · 2022Review
- Platelet-Rich Fibrin Increases BMP2 Expression in Oral Fibroblasts via Activation of TGF-β Signaling.International journal of molecular sciences · 2021Article
- Granulocytes and their Involvement in the Foreign Body Response to Biomaterials and Tissue Repair.In vivo (Athens, Greece)Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leukocyte-platelet rich fibrin (L-PRF) is extensively used in the dentistry field and other clinical scenarios due to its regeneration properties. The goal of the present study was to depict the L-PRF secretome and how it changes over time. We obtained L-PRF membranes and cultured them in DMEM. The secretome was collected at days 3, 7 and 21. The secretome at day 3 was analysed by LC-MS/MS and differences over time were analysed by Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH). Overall, 705 proteins were identified in the secretome of L-PRF membranes after 3 days of culture, including growth factors (EGF, PDGFA) and proteins related to platelet and neutrophil degranulation. A total of 202 differentially secreted proteins were quantified by SWATH when comparing secretomes at days 3, 7 and 21. Most of them were enriched at day 3 such as MMP9, TSP1 and CO3. On the contrary, fibrinogen and CATS were found down-regulated at day 3. Growth factor and western blotting analysis corroborated the proteomic results. This is the most detailed proteome analysis of the L-PRF secretome to date. Proteins and growth factors identified, and their kinetics, provide novel information to further understand the wound healing properties of L-PRF.
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Registered trials
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