Evidence map›Paper›PMID 32883809›Full record

ArticleThe Journal of biological chemistry2020

A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits.

Samantha J Mascuch, Sara Fakhretaha-Aval, Jessica C Bowman, Minh Thu H Ma, Gwendell Thomas, Bettina Bommarius, Chieri Ito, Liangjun Zhao, Gary P Newnam, Kavita R Matange and 25 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. iScience · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Maximising the Use of Scarce qPCR Master Mixes.International journal of molecular sciences · 2022
    Article
  12. Article
  13. Review
  14. Detection of SARS-CoV-2 by Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  15. Article
  16. Homebrew reagents for low-cost RT-LAMP.Journal of biomolecular techniques : JBT · 2021
    Article
  17. Article
  18. Homebrew reagents for low cost RT-LAMP.medRxiv : the preprint server for health sciences · 2021
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

35 authors.

Samantha J MascuchSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Sara Fakhretaha-AvalSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Jessica C BowmanSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Minh Thu H MaSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Gwendell ThomasSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Bettina BommariusPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Chieri ItoSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Liangjun ZhaoSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Gary P NewnamSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Kavita R MatangeSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Hem R ThapaSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Brett BarlowSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Rebecca K DoneganSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Nguyet A NguyenSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Emily G SaccuzzoSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Chiamaka T ObianyorSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Suneesh C KarunakaranSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Pamela PolletSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Brooke Rothschild-MancinelliSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Santi Mestre-FosSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Rebecca Guth-MetzlerSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Anton V BryksinPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Anton S PetrovSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Mallory HazellSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
Carolyn B IbbersonSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Petar I PenevSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Robert G ManninoWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Wilbur A LamWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA; Institute for Electronics and Nanotechnology, Georgia Institute of Technology, Atlanta, Georgia, USA; Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, Atlanta, Georgia, USA; Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA.
Andrés J GarciaPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA; School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Julia KubanekSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Vinayak AgarwalSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Nicholas V HudSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Jennifer B GlassPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA; School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA. Electronic address: covid-test-support@gatech.edu.
Loren Dean WilliamsSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.
Raquel L LiebermanSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA.

Funding

Technology Training and Dissemination CoreU54EB027690 · NIBIB · EMORY UNIVERSITY · PI Wilbur A Lam, GREGORY S MARTIN · 2018 to 2026
$96.1M
NIBIB NIH HHS U54 EB027690
6 · The paper itself

Abstract

Widespread testing for the presence of the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise, and/or instrumentation necessary to detect the virus by quantitative RT-PCR (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably with a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings.

Indexed as

Technology TransferBiotechnologyCOVID-19COVID-19 Nucleic Acid TestingHumansReagent Kits, DiagnosticReal-Time Polymerase Chain ReactionSARS-CoV-2UniversitiesReagent Kits, DiagnosticcoronavirusDNA polymeraseformulationinfectious diseasepolymerase chain reaction (PCR)protein purificationreverse transcriptasereverse transcriptionribonuclease inhibitorRNART-qPCRSARS-CoV-2virus

Identifiers

PMID32883809
PMCPMC7667971

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.