Evidence map›Paper›PMID 32883361›Full record

ArticleBMC research notes2020

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.).

Catalina Restrepo-Osorio, Alejandro Gil-Correal, Lina Chamorro-Gutiérrez, Viviana Ramírez-Ríos, Javier C Álvarez, Diego Villanueva-Mejía

Abstract read
In one paragraph

Article in BMC research notes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Catalina Restrepo-OsorioDepartment of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia.
Alejandro Gil-CorrealDepartment of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia.
Lina Chamorro-GutiérrezDepartment of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia.
Viviana Ramírez-RíosDepartment of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia.
Javier C ÁlvarezDepartment of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia.
Diego Villanueva-MejíaDepartment of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia. dvillanu@eafit.edu.co.ORCID http://orcid.org/0000-0002-3837-5006

Funding

Sistema General de Regalías de Colombia 4600 000480
6 · The paper itself

Abstract

objectiveIt is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species.

resultsShoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species.

Indexed as

EuphorbiaceaeFatty Acids, UnsaturatedFatty Acids, UnsaturatedAcclimatizationAdventitious shootsOilseedRootingTissue culture

Identifiers

PMID32883361
PMCPMC7650214

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.