ArticlePLoS neglected tropical diseases2020
Toward implementation of combined incompatible and sterile insect techniques for mosquito control: Optimized chilling conditions for handling Aedes albopictus male adults prior to release.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Effects of chilling, fluorescent dust marking, and X-ray irradiation on the quality of male Anopheles arabiensis for sterile insect technique applications.Scientific reports · 2026Article
- Suitability of a chilled environmental box of the Precision X-RAD 320 cabinet style irradiator for the irradiation of mosquitoes and tsetse in the context of the sterile insect technique.Journal of economic entomology · 2026Article
- Field implementation of the sterile insect technique against Aedes aegypti in Recife, Brazil: operational challenges and impact of release frequency on vector dynamics.Infectious diseases of poverty · 2026Article
- Stress in dipteran insects mass-reared for sterile insect technique applications.Insect science · 2025Review
- Ecological dynamics of field Aedes albopictus populations under Wolbachia-mediated suppression.Infectious diseases of poverty · 2025Article
- Evaluating the performance of three packing methods for long-distance transport of sterile adult Aedes albopictus males.Scientific reports · 2025Article
- Mating harassment may boost the effectiveness of the sterile insect technique for Aedes mosquitoes.Nature communications · 2024Article
- Mass irradiation of adult Aedes mosquitoes using a coolable 3D printed canister.Scientific reports · 2024Article
- Release of sterile Aedes aegypti mosquitoes: chilling effect on mass-reared males survival and escape ability and on irradiated males sexual competitiveness.Scientific reports · 2023Article
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- Radiation dose-fractionation in adult Aedes aegypti mosquitoes.Parasite (Paris, France) · 2023Article
- A standalone incompatible insect technique enables mosquito suppression in the urban subtropics.Communications biology · 2022Article
- Exploring Conditions for Handling Packing and ShippingInsects · 2022Article
- Current Status of Mosquito Handling, Transporting and Releasing in Frame of the Sterile Insect Technique.Insects · 2022Review
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- Effects of Chilling and Anoxia on the Irradiation Dose-Response in AdultFrontiers in bioengineering and biotechnology · 2022Article
- Response of male adultFrontiers in bioengineering and biotechnology · 2022Article
- Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies.Tropical medicine and infectious disease · 2020Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Combined incompatible and sterile insect technique (IIT-SIT) has been considered to be an effective and safe approach to control mosquito populations. Immobilization of male adults by chilling is a crucial process required for the packing, transportation and release of the mosquitoes during the implementation of IIT-SIT for mosquito control. In this study, effects of chilling on the Aedes albopictus males with triple Wolbachia infections (HC line), a powerful weapon to fight against the wild type Ae. albopictus population via IIT-SIT, were evaluated under both laboratory and field conditions. Irradiated HC (IHC) males were exposed to 1, 5 and 10°C for 1, 2, 3, 6 and 24 h. The survival rate of the post-chilled IHC males was then monitored. Longevity of post-chilled IHC males was compared to non-chilled males under laboratory and semi-field conditions. Mating competitiveness of IHC/HC males after exposure to 5 or 10°C for 0, 3 and 24 h was then evaluated. Effects of compaction and transportation under chilled conditions on the survival rate of IHC males were also monitored. The optimal chilling conditions for handling IHC males were temperatures between 5 and 10°C for a duration of less than 3 h with no negative impacts on survival rate, longevity and mating competitiveness when compared to non-chilled males. However, the overall quality of post-chilled IHC/HC males decreased when exposed to low temperatures for 24 h. Reduced survival was observed when IHC males were stored at 5°C under a compaction height of 8 cm. Transportation with chilling temperatures fluctuating from 8 to 12°C has no negative impact on the survival of IHC males. This study identified the optimal chilling temperature and duration for the handling and transportation of Ae. albopictus IHC male adults without any detrimental effect on their survival, longevity and mating competitiveness. Further studies are required to develop drone release systems specific for chilled mosquitoes to improve release efficiency, as well as to compare the population suppression efficiency between release of post-chilled and non-chilled males in the field.
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