Evidence map›Paper›PMID 32881143›Full record

ArticleClinical oral implants research2020

Bone formation around unstable implants is enhanced by a WNT protein therapeutic in a preclinical in vivo model.

Benjamin R Coyac, Brian Leahy, Zhijun Li, Giuseppe Salvi, Xing Yin, John B Brunski, Jill A Helms

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical oral implants research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Trial
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Benjamin R CoyacDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.ORCID https://orcid.org/0000-0003-0105-6703
Brian LeahyDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.
Zhijun LiDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.
Giuseppe SalviDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.
Xing YinDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.
John B BrunskiDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.
Jill A HelmsDepartment of Plastic and Reconstructive Surgery, School of Medicine, Stanford University, Palo Alto, CA, USA.ORCID https://orcid.org/0000-0002-0463-396X
Stanford University · US

Funding

Mechanobiology at Healing Bone-Implant InterfacesR01DE024000 · NIDCR · STANFORD UNIVERSITY · PI BRUNSKI, JOHN BEYER, HELMS, JILL A · 2014 to 2018
$3.4M
AO Foundation UDQAM to J.A.HNIDCR NIH HHS R01 DE024000
6 · The paper itself

Abstract

objectivesOur objective was to test the hypothesis that local delivery of a WNT protein therapeutic would support osseointegration of an unstable implant placed into an oversized osteotomy and subjected to functional loading. MATERIALS AND

methodsUsing a split-mouth design in an ovariectomized (OVX) rat model, 50 titanium implants were placed in oversized osteotomies. Implants were subjected to functional loading. One-half of the implants were treated with a liposomal formulation of WNT3A protein (L-WNT3A); the other half received an identical liposomal formulation containing phosphate-buffered saline (PBS). Finite element modeling estimated peri-implant strains caused by functional loading. Histological, molecular, cellular, and quantitative micro-computed tomographic (µCT) imaging analyses were performed on samples from post-implant days (PID) 3, 7, and 14. Lateral implant stability was quantified at PID 7 and 14.

resultsFinite element analyses predicted levels of peri-implant strains incompatible with new bone formation. Micro-CT imaging, histological, and quantitative immunohistochemical (IHC) analyses confirmed that PBS-treated implants underwent fibrous encapsulation. In those cases where the peri-implant environment was treated with L-WNT3A, µCT imaging, histological, and quantitative IHC analyses demonstrated a significant increase in expression of proliferative (PCNA) and osteogenic (Runx2, Osterix) markers. One week after L-WNT3A treatment, new bone formation was evident, and two weeks later, L-WNT3A-treated gaps had a stiffer interface compared to PBS-treated gaps.

conclusionIn a rat model, unstable implants undergo fibrous encapsulation. If the same unstable implants are treated with L-WNT3A at the time of placement, then it results in significantly more peri-implant bone and greater interfacial stiffness.

Indexed as

Dental ImplantsOsteogenesisAnimalsOsseointegrationRatsTitaniumWnt ProteinsDental ImplantsTitaniumWnt Proteinsdentalimplant failureoralosseointegrationstability

Identifiers

PMID32881143
PMCPMC7722236
OpenAlexW3082602697

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.