Evidence map›Paper›PMID 32880047›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2021

Expression of p27 and p16 and their clinical significance in gastric cancer.

S Kumari, P Kumar, M Kumar, S Singh, G Narayan

Abstract read
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In one paragraph

Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 13 citations in OpenAlex.

  1. p27 Expression in Wild-Type KRAS Colon Cancer.Journal of cellular and molecular medicine · 2026
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  10. Role of Genetic Variations inCancer genomics & proteomics
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

S KumariCancer Genetics Laboratory, Department of Molecular and Human Genetics, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
P KumarDepartment of Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India. puneetimsbhu@gmail.com.ORCID http://orcid.org/0000-0001-5378-1988
M KumarDepartment of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
S SinghDepartment of Zoology, Banaras Hindu University, Mahila Mahavidyalaya, Varanasi, 221 005, India.
G NarayanCancer Genetics Laboratory, Department of Molecular and Human Genetics, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Banaras Hindu University · INInstitute of Medical Sciences · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDeregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. p27

methodsWe recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity.

resultsWe demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level.

conclusionsThe present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients.

Indexed as

AdultBiomarkers, TumorCell Line, TumorCyclin-Dependent Kinase Inhibitor p16Cyclin-Dependent Kinase Inhibitor p27Disease ProgressionEarly Detection of CancerFemaleFluorescent Antibody TechniqueG1 Phase Cell Cycle CheckpointsHumansImmunohistochemistryMaleMiddle AgedReverse Transcriptase Polymerase Chain ReactionRNA, MessengerBiomarkers, TumorCDKN1B protein, humanCyclin-Dependent Kinase Inhibitor p16Cyclin-Dependent Kinase Inhibitor p27RNA, MessengerDiagnostic biomarkerExpression profileGastric cancerp16p27

Identifiers

PMID32880047
OpenAlexW3081950411

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.