ArticleNature communications2020
Evolution of a guarded decoy protease and its receptor in solanaceous plants.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Apoplast Engineering Unveiled: Multi-Target Strategies to Disarm Pathogens and Elevate Global Food Security.Plant, cell & environment · 2026Review
- A secreted citrus protease cleaves an outer membrane protein of the Huanglongbing pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Pathogen-inspired engineering of plant protease enhances late blight resistance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Silenced cutters: mechanisms and effects of protease inhibition in plant-pathogen interactions.Journal of experimental botany · 2025Review
- Hacking the immune system: plant immune protease engineering for crop protection.Journal of experimental botany · 2025Review
- Learning the language of plant immunity: opportunities and challenges for AI-assisted modelling of fungal effector x host protein complexes.Computational and structural biotechnology journal · 2025Review
- A disease resistance protein triggers oligomerization of its NLR helper into a hexameric resistosome to mediate innate immunity.Science advances · 2024Article
- Dual transcriptional characterization of spinach and Peronospora effusa during resistant and susceptible race-cultivar interactions.BMC genomics · 2024Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Article
- Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.The Plant cell · 2024Article
- The NRC0 gene cluster of sensor and helper NLR immune receptors is functionally conserved across asterid plants.The Plant cell · 2024Article
- Recreating or Rcr3ating? Bioengineering homologs of the Rcr3 protease to activate plant immunity.The Plant cell · 2024Article
- Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.Scientific reports · 2024Article
- Enhanced late blight resistance by engineering an EpiC2B-insensitive immune protease.Plant biotechnology journal · 2024Article
- Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen Infection.Plant & cell physiology · 2024Article
- AlphaFold-Multimer predicts cross-kingdom interactions at the plant-pathogen interface.Nature communications · 2023Article
- Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors.Nature communications · 2023Article
- The function of plant PR1 and other members of the CAP protein superfamily in plant-pathogen interactions.Molecular plant pathology · 2023Review
- Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants.Molecular plant pathology · 2023Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Rcr3 is a secreted protease of tomato that is targeted by fungal effector Avr2, a secreted protease inhibitor of the fungal pathogen Cladosporium fulvum. The Avr2-Rcr3 complex is recognized by receptor-like protein Cf-2, triggering hypersensitive cell death (HR) and disease resistance. Avr2 also targets Rcr3 paralog Pip1, which is not required for Avr2 recognition but contributes to basal resistance. Thus, Rcr3 acts as a guarded decoy in this interaction, trapping the fungus into a recognition event. Here we show that Rcr3 evolved > 50 million years ago (Mya), whereas Cf-2 evolved <6Mya by co-opting the pre-existing Rcr3 in the Solanum genus. Ancient Rcr3 homologs present in tomato, potato, eggplants, pepper, petunia and tobacco can be inhibited by Avr2 with the exception of tobacco Rcr3. Four variant residues in Rcr3 promote Avr2 inhibition, but the Rcr3 that co-evolved with Cf-2 lacks three of these residues, indicating that the Rcr3 co-receptor is suboptimal for Avr2 binding. Pepper Rcr3 triggers HR with Cf-2 and Avr2 when engineered for enhanced inhibition by Avr2. Nicotiana benthamiana (Nb) is a natural null mutant carrying Rcr3 and Pip1 alleles with deleterious frame-shift mutations. Resurrected NbRcr3 and NbPip1 alleles were active proteases and further NbRcr3 engineering facilitated Avr2 inhibition, uncoupled from HR signalling. The evolution of a receptor co-opting a conserved pathogen target contrasts with other indirect pathogen recognition mechanisms.
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