Evidence map›Paper›PMID 32878950›Full record

ArticleJournal of clinical microbiology2020

Targeted Metagenomics for Clinical Detection and Discovery of Bacterial Tick-Borne Pathogens.

Luke Kingry, Sarah Sheldon, Stephanie Oatman, Bobbi Pritt, Melissa Anacker, Jenna Bjork, David Neitzel, Anna Strain, Jon Berry, Lynne Sloan and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Lyme borreliosis.Nature reviews. Disease primers · 2026
    Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Open forum infectious diseases · 2022
    Article
  14. An Update on the Laboratory Diagnosis ofPathogens (Basel, Switzerland) · 2021
    Review
  15. Article
  16. Article
  17. Detection ofFrontiers in veterinary science · 2021
    Article
  18. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Luke Kingry *Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA vtx8@cdc.gov.ORCID 0000-0002-5724-2575
Sarah Sheldon *Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Stephanie Oatman *Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Bobbi PrittDivision of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Melissa AnackerMinnesota Department of Health, St. Paul, Minnesota, USA.
Jenna BjorkMinnesota Department of Health, St. Paul, Minnesota, USA.
David NeitzelMinnesota Department of Health, St. Paul, Minnesota, USA.
Anna StrainMinnesota Department of Health, St. Paul, Minnesota, USA.
Jon BerryDivision of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Lynne SloanDivision of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota, USA.
Laurel Respicio-KingryDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Elizabeth DietrichDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Karen BlochVanderbilt University Medical Center, Nashville, Tennessee, USA.
Abelardo MoncayoTennessee Department of Health, Nashville, Tennessee, USA.
Ganesh SrinivasamoorthyScientific Computing and Bioinformatics Support, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Bin HuScientific Computing and Bioinformatics Support, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Alison HinckleyDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Paul MeadDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Kiersten KugelerDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
Jeannine PetersenDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tick-borne illness and >1,000 controls. Taxonomic predictions for tick-borne bacteria were exceptionally accurate, as independently validated by secondary testing. Overall, 881 specimens were positive for bacterial tick-borne agents. Twelve tick-borne bacterial species were detected, including two novel pathogens, representing a 100% increase in the number of tick-borne bacteria identified compared to what was possible by initial PCR testing. In three blood specimens, two tick-borne bacteria were simultaneously detected. Seven bacteria, not known to be tick transmitted, were also confirmed to be unique to samples from persons suspected of having tick-borne illness. These results indicate that 16S V1-V2 metagenomics can greatly simplify diagnosis and accelerate the discovery of bacterial tick-borne pathogens.

Indexed as

EhrlichiosisTick-Borne DiseasesTicksAnimalsBacteriaHumansMetagenomicsRNA, Ribosomal, 16SRNA, Ribosomal, 16Sanaplasmosishuman granulocytic ehrlichiosisLyme diseasemetagenomicstick-borne bacteriavector-borne diseases

Identifiers

PMID32878950
PMCPMC7587092

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.