Evidence map›Paper›PMID 32878681›Full record

ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2020

Cryopreservation of Mauritian Cynomolgus Macaque (

Nick S Strelchenko, Jenna Kropp Schmidt, Katherine D Mean, Michele L Schotzko, Thaddeus G Golos, Igor I Slukvin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Nick S StrelchenkoWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin.
Jenna Kropp SchmidtWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin.
Katherine D MeanWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin.
Michele L SchotzkoWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin.
Thaddeus G GolosWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin; Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, Wisconsin; Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin.
Igor I SlukvinWisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin; Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;, Email: islukvin@wisc.edu.
University of Wisconsin–Madison · US

Funding

WNPRC Supplemental Request for Nonhuman Primate Enclosures to Equip HIV/AIDS-Related Research FacilitiesP51OD011106 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI Dorota A. Grejner-Brzezinska · 2012 to 2026
$150.7M
EXTRAMURAL RES FACIL CONST: REPRODUCTIVE, GROWTH &DVMT, MENOPAUSEC06RR020141 · NCRR · UNIVERSITY OF WISCONSIN MADISON · PI CADWALLADER, MARTIN · 2004 to 2004
$3.6M
CCR5-mutant monkey model to facilitate the development of novel stem cell-based therapies for AIDSR24OD021322 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI GOLOS, THADDEUS G, SLUKVIN, IGOR I. · 2016 to 2019
$3.3M
EXTRAMURAL REGIONAL FACILITIES CONSTRUCTIONC06RR015459 · NCRR · UNIVERSITY OF WISCONSIN MADISON · PI CADWALLADER, MARTIN · 2000 to 2000
$1.9M
Primate-specific miRNAs in Embryonic and Placental DevelopmentK99HD099154 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI SCHMIDT, JENNA ANN · 2019 to 2021
$228k
NCRR NIH HHS C06 RR015459NCRR NIH HHS C06 RR020141NICHD NIH HHS K99 HD099154NIH HHS P51 OD011106NIH HHS R24 OD021322
6 · The paper itself

Abstract

The objective of this study was to optimize cryopreservation of sperm from Mauritian cynomolgus macaques (MCM) in defined conditions. Sperm viability and motility were compared between sperm cryopreserved in chemically-defined freezing media with variable osmolarity and the presence of either ethylene glycol or glycerol. The highest percentage viability (after freeze-thaw) was seen in sperm samples that were cryopreserved in medium with an osmolarity of 310 mOsm, while higher osmolarities markedly decreased sperm viability. Ethylene glycol and glycerol at concentrations of 4.6% and 5%, respectively, preserved sperm viability to an equivalent degree. Although higher motility rates and higher straight-line velocities were observed in sperm samples frozen in glycerol compared with ethylene glycol, these differences were not statistically significant. Thawed sperm frozen in defined conditions with glycerol were capable of fertilizing MCM oocytes in vitro, with development to the blastocyst stage. The protocol described here provides an effective method for cryopreservation of sperm to facilitate subsequent in vitro fertilization and genome editing of embryos in MCM species.

Indexed as

Macaca fascicularisAnimalsCryopreservationCryoprotective AgentsEthylene GlycolFemaleFertilization in VitroGlycerolMaleSemen PreservationSpermatozoaCryoprotective AgentsEthylene GlycolGlycerol

Identifiers

PMID32878681
PMCPMC7604693
OpenAlexW3083519391

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.