ArticleActa neuropathologica1988
Glial fibrillary acidic protein, vimentin and fibronectin in primary cultures of human glioma and fetal brain.
Article in Acta neuropathologica, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- From Classical to Emerging Biomarkers of Brain and Central Nervous System Tumors. An Evidence-Based Review with a Focus on Gliomas.Cellular and molecular neurobiology · 2026Review
- Slow Off-Rate Modified Aptamer (SOMAmer) Proteomic Analysis of Patient-Derived Malignant Glioma Identifies Distinct Cellular Proteomes.International journal of molecular sciences · 2021Article
- Biology of Glioblastoma Multiforme-Exploration of Mitotic Catastrophe as a Potential Treatment Modality.International journal of molecular sciences · 2020Review
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Expression of different extracellular matrix components in human brain tumor and melanoma cells in respect to variant culture conditions.Journal of neuro-oncology · 1999Article
- Morphologic, immunologic, biochemical, and cytogenetic characteristics of the human glioblastoma-derived cell line, SNB-19.In vitro cellular & developmental biology. Animal · 1995Article
- Products of cells from gliomas: IX. Evidence that two fundamentally different mechanisms change extracellular matrix expression by gliomas.Journal of neuro-oncology · 1995Article
- GFAP expression in the subcutaneous tumors of immature glial cell line (HITS glioma) derived from ENU-induced rat glioma.Journal of neuro-oncology · 1993Article
- Ultrastructural characteristics of glial fibrillary acidic protein expression in epoxy resin-embedded human brain tumors.Acta neuropathologica · 1993Article
- Vimentin immunoreactivity in normal and pathological human brain tissue.Acta neuropathologica · 1992Article
- Expression of fibronectin and laminin by different types of mouse glial cells cultured in a serum-free medium.Cytotechnology · 1991Article
- A cell line of human malignant astrocytoma producing autocrine growth factor.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1991Article
- Evolution of several cytoskeletal proteins of astrocytes in primary culture: effect of prenatal alcohol exposure.Neurochemical research · 1991Article
- Morphological, immunocytochemical and growth characteristics of three human glioblastomas established in vitro.Virchows Archiv. A, Pathological anatomy and histopathology · 1991Article
- A cerebral glioma model for experimental therapy and in vivo invasion studies in syngeneic BD IX rats.Journal of neuro-oncology · 1990Article
- Contrary effect of lactic acid on expression of neuron-specific enolase and glial fibrillary acidic protein in human glioma cells.Acta neuropathologica · 1990Article
- Immunohistochemical localisation of tissue plasminogen activator in human brain tumours.British journal of cancer · 1989Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of glial fibrillary acidic protein (GFAP), vimentin and fibronectin (Fn) was studied in cells cultured from human glioma and fetal brain by indirect immunofluorescence (IIF) microscopy and multiple labelling experiments. In the primary cultures a major part (20%-70%) of the cells usually displayed both GFAP and vimentin and the rest of the cells only vimentin. A prominent variation in GFAP and vimentin fluorescence intensity sometimes made interpretation of double IIF stainings difficult. However, occasional GFAP-positive cells appeared vimentin negative in primary glioma cultures, whereas in fetal brain primary cultures cells that were preferentially GFAP positive also showed at least a weak vimentin immunoreactivity. Only a fraction of the cells, roughly corresponding to the GFAP-negative cells, were Fn positive in the primary cultures. As judged by double IIF, the GFAP-positive cells were usually Fn negative, while the Fn-positive cells were vimentin positive. This could also be demonstrated in triple IIF experiments. During serial subcultivation the amount of cells expressing GFAP decreased, while the number of Fn-positive cells increased. By the third to fourth passage GFAP positivity was usually lost, all cells expressed vimentin and most cells also Fn. The results of the present study demonstrate a general coexpression of GFAP and vimentin in cultured astroglial cells, in addition to cells expressing only vimentin. Interestingly, occasional glioma cells seem to contain GFAP as the only intermediate filament protein as detected by immunocytochemistry.(ABSTRACT TRUNCATED AT 250 WORDS)
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