ArticleFrontiers of medicine2021
Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes.
Article in Frontiers of medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis.International journal of molecular sciences · 2023Pooled it
- The effect of extended cryo-storage following vitrification on embryo competence: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2022Pooled it
- Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses.Journal of ovarian research · 2022Trial
- Influencing factors of live birth in vitrified oocyte donation cycles: A retrospective cohort study.European journal of obstetrics & gynecology and reproductive biology: X · 2026Article
- Identification of cryosensitive niches and a targetable FOS/AP‑1 program in the human ovarian cortex by single‑cell and spatial transcriptomics.BMC medicine · 2026Article
- Dual-omics reveals temporal translational recovery landscapes and cryodamage repair mechanisms in vitrified mouse oocytes.Journal of assisted reproduction and genetics · 2025Article
- Association Between Human Embryo Culture Conditions, Cryopreservation, and the Potential Risk of Birth Defects in Children Conceived Through Assisted Reproduction Technology.Medicina (Kaunas, Lithuania) · 2025Review
- Current status and hotspots of in vitro oocyte maturation: a bibliometric study of the past two decades.Journal of assisted reproduction and genetics · 2025Review
- Cryopreservation, cryoprotectants, and potential risk of epigenetic alteration.Journal of assisted reproduction and genetics · 2024Review
- Vitrification of human blastocysts for couples undergoing assisted reproduction: an updated review.Frontiers in cell and developmental biology · 2024Review
- Article
- Can Cryopreservation in Assisted Reproductive Technology (ART) Induce Epigenetic Changes to Gametes and Embryos?Journal of clinical medicine · 2023Review
- Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation.International journal of molecular sciences · 2023Article
- The impact of zygote vitrification timing on pregnancy rate in frozen-thawed IVF/ICSI cycles.Frontiers in cell and developmental biology · 2023Article
- Article
- Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension.Journal of animal science and biotechnology · 2022Article
- Vitrification of Pronuclear Zygotes Perturbs Porcine Zygotic Genome Activation.Animals : an open access journal from MDPI · 2022Article
- DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development.Porcine health management · 2021Article
- Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes.Animals : an open access journal from MDPI · 2021Review
- Cryopreservation of Gametes and Embryos and Their Molecular Changes.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30-32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1,2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.