Evidence map›Paper›PMID 32876583›Full record

ArticleJournal of synchrotron radiation2020

Current status and future opportunities for serial crystallography at MAX IV Laboratory.

Anastasya Shilova, Hugo Lebrette, Oskar Aurelius, Jie Nan, Martin Welin, Rebeka Kovacic, Swagatha Ghosh, Cecilia Safari, Ross J Friel, Mirko Milas and 10 more

Open access · hybridAbstract read
In one paragraph

Article in Journal of synchrotron radiation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 21 citations in OpenAlex.

  1. Jungfraujoch: 38 GB sJournal of applied crystallography · 2026
    Article
  2. Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026
    Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes.Acta crystallographica. Section D, Structural biology · 2023
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 7 institutions in 2 countries.

Anastasya ShilovaMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.ORCID 0000-0002-5931-5790
Hugo LebretteDepartment of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16 C, Stockholm 10691, Sweden.ORCID 0000-0002-8081-181X
Oskar AureliusMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.
Jie NanMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.
Martin WelinSARomics Biostructures, Medicon Village, Scheeletorget 1, Lund 22363, Sweden.ORCID 0000-0001-5786-1600
Rebeka KovacicSARomics Biostructures, Medicon Village, Scheeletorget 1, Lund 22363, Sweden.
Swagatha GhoshDepartment of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 40530, Sweden.ORCID 0000-0003-1063-0674
Cecilia SafariDepartment of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 40530, Sweden.
Ross J FrielSchool of Information Technology, Halmstad University, Halmstad 30118, Sweden.ORCID 0000-0002-0480-4079
Mirko MilasMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.
Zdenek MatejMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.ORCID 0000-0002-2585-573X
Martin HögbomDepartment of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius väg 16 C, Stockholm 10691, Sweden.ORCID 0000-0001-5574-9383
Gisela BrändénDepartment of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 40530, Sweden.ORCID 0000-0001-5615-7187
Marco KloosEuropean XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
Robert L ShoemanDepartment of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.ORCID 0000-0002-1030-6738
Bruce DoakDepartment of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
Thomas UrsbyMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.ORCID 0000-0001-7371-5507
Maria HåkanssonSARomics Biostructures, Medicon Village, Scheeletorget 1, Lund 22363, Sweden.ORCID 0000-0002-7264-6316
Derek T LoganSARomics Biostructures, Medicon Village, Scheeletorget 1, Lund 22363, Sweden.ORCID 0000-0002-0098-8560
Uwe MuellerMAX IV Laboratory, Lund University, Fotongatan 2, Lund 22484, Sweden.ORCID 0000-0002-7139-0718
Lund University · SEMedicon Village · SEUniversity of Gothenburg · SEMax Planck Institute for Medical Research · DEStockholm University · SEEuropean X-Ray Free-Electron Laser · DEHalmstad University · SE

Funding

VINNOVA N 2018-04409
6 · The paper itself

Abstract

Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.

Indexed as

SynchrotronsCrystallography, X-RayEquipment DesignLaboratoriesSilicon CompoundsSwedenTemperatureSilicon Compoundssilicon nitridehigh-viscosity injectorsmacromolecular crystallographysample deliveryserial crystallography

Identifiers

PMID32876583
PMCPMC7467353
OpenAlexW3080539147

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.