ArticleChemical science2020
A photo zipper locked DNA nanomachine with an internal standard for precise miRNA imaging in living cells.
Article in Chemical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 87 citations in OpenAlex.
- Light-responsive DNA nanostructures.Nature reviews. Chemistry · 2026Review
- An Upconversion Fluorescent Resonant Energy Transfer Biosensor for the Detection of microRNA through DNA Hybridization.ACS omega · 2024Article
- Luminescent photon-upconversion nanoparticles with advanced functionalization for smart sensing and imaging.Mikrochimica acta · 2024Review
- A "dual-key-and-lock" DNA nanodevice enables spatially controlled multimodal imaging and combined cancer therapy.Chemical science · 2024Article
- Phototriggered Equilibrated and Transient Orthogonally Operating Constitutional Dynamic Networks Guiding Biocatalytic Cascades.Journal of the American Chemical Society · 2024Article
- Review
- Dynamic Fusion of Nucleic Acid Functionalized Nano-/Micro-Cell-Like Containments: From Basic Concepts to Applications.ACS nano · 2023Review
- PhotocleavableChemical reviews · 2023Review
- Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis.Acta pharmaceutica Sinica. B · 2023Review
- Light responsive nucleic acid for biomedical application.Exploration (Beijing, China) · 2022Review
- Biologically stable threose nucleic acid-based probes for real-time microRNA detection and imaging in living cells.Molecular therapy. Nucleic acids · 2022Article
- A core-brush 3D DNA nanostructure: the next generation of DNA nanomachine for ultrasensitive sensing and imaging of intracellular microRNA with rapid kinetics.Chemical science · 2021Article
- Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.Chemical science · 2021Article
- miR122-controlled all-in-one nanoplatform forMaterials today. Bio · 2021Article
- Microfluidic synthesis of high-valence programmable atom-like nanoparticles for reliable sensing.Chemical science · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA nanomachines are capable of converting tiny triggers into autonomous accelerated cascade hybridization reactions and they have been used as a signal amplification strategy for intracellular imaging. However, the "always active" property of most DNA nanomachines with an "absolute intensity-dependent" signal acquisition mode results in "false positive signal amplification" by extracellular analytes and impairs detection accuracy. Here we design a photo zipper locked miRNA responsive DNA nanomachine (PZ-DNA nanomachine) based on upconversion nanoparticles (UCNPs) with a photo-cleavable DNA strand to block the miRNA recognition region, which provided sufficient protection to the DNA nanomachine against nonspecific extracellular activation and allowed satisfactory signal amplification for sensitive miRNA imaging after intracellular photoactivation. Multiple emissions from the UCNPs were also utilized as an internal standard to self-calibrate the intracellular miRNA responsive fluorescence signal. The presented PZ-DNA nanomachine demonstrated the sensitive imaging of intracellular miRNA from different cell lines, which resulted in good accordance with qRT-PCR measurements, providing a universal platform for precise imaging in living cells with high spatial-temporal specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.