ArticleChemical science2020
Determination of protein-ligand binding modes using fast multi-dimensional NMR with hyperpolarization.
Article in Chemical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Proton signal enhancement of three orders of magnitude in high-field liquid-state NMR.Nature communications · 2026Article
- Quantification of Ligand-Membrane Interactions Using DNP-NMR Relaxometry.Analytical chemistry · 2026Article
- Ultrafast Nuclear Magnetic Resonance as a Tool to Detect Rapid Chemical Change in Solution.ACS physical chemistry Au · 2024Review
- Cross-Polarization of Insensitive Nuclei from Water Protons for Detection of Protein-Ligand Binding.Journal of the American Chemical Society · 2024Article
- Solution NMR investigations of integral membrane proteins: Challenges and innovations.Current opinion in structural biology · 2023Review
- Biomolecular interactions studied by low-field NMR using SABRE hyperpolarization.Chemical science · 2023Article
- Parahydrogen in Reversible Exchange Induces Long-LivedAnalytical chemistry · 2023Article
- Nuclear Overhauser spectroscopy in hyperpolarized water - chemicalChemical communications (Cambridge, England) · 2022Article
- Tools shaping drug discovery and development.Biophysics reviews · 2022Review
- Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration.Journal of the American Chemical Society · 2022Article
- Hyperpolarized water as universal sensitivity booster in biomolecular NMR.Nature protocols · 2022Review
- Interfacing Liquid State Hyperpolarization Methods with NMR Instrumentation.Journal of magnetic resonance open · 2022Article
- Room-temperature dynamic nuclear polarization enhanced NMR spectroscopy of small biological molecules in water.Nature communications · 2021Article
- Application of Relaxation Dispersion of HyperpolarizedAngewandte Chemie (International ed. in English) · 2021Article
- Characterization of protein-ligand interactions by SABRE.Chemical science · 2021Article
- Challenges and Advances in the Application of Dynamic Nuclear Polarization to Liquid-State NMR Spectroscopy.The journal of physical chemistry. B · 2021Review
- Dynamic Nuclear Polarization Using 3D Aromatic Boron Cluster Radicals.The journal of physical chemistry letters · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidation of small molecule-protein interactions provides essential information for understanding biological processes such as cellular signaling, as well as for rational drug development. Here, multi-dimensional NMR with sensitivity enhancement by dissolution dynamic nuclear polarization (D-DNP) is shown to allow the determination of the binding epitope of folic acid when complexed with the target dihydrofolate reductase. Protein signals are selectively enhanced by polarization transfer from the hyperpolarized ligand. A pseudo three-dimensional data acquisition with ligand-side Hadamard encoding results in protein-side [
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Registered trials
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