ArticlePlant physiology2020
Dynamic Control of the High-Affinity Iron Uptake Complex in Root Epidermal Cells.
Article in Plant physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 115 citations in OpenAlex.
- Meta-Analysis of Iron Excess Stress in Rice: Genes and Mechanisms of Tolerance to Acidic Soil.Physiologia plantarumPooled it
- TurboID-based proteomic profiling reveals proxitome of the IRT1 metal transporter and new insight into IRT1 endocytosis.The New phytologist · 2026Article
- Hypoxic and Fe-Responses are Regulated by the ERFVII Factors and the PCO Branch of the N-Degron Pathway According to Iron Availability.Plant, cell & environment · 2026Article
- The Arabidopsis NPF7.2 mediates coumarin uptake for root iron acquisition.The New phytologist · 2026Article
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
- Physiological and molecular responses of colored wheat seedlings under varying nutrient stress conditions.Protoplasma · 2026Article
- Isolation of Siderophore-Producing Bacteria from Extreme Environments and Their Role in Improving Maize Salinity-Alkalinity Tolerance.Microorganisms · 2026Article
- Root cell wall plasticity in iron homeostasis: an overlooked frontier in plant nutrition.The New phytologist · 2026Review
- Iron need attention: a review on the role of iron in plants.Frontiers in plant science · 2026Review
- Canonical tyrosine-based motifs are required for constitutive endocytosis and polarity of IRT1 and contribute to metal uptake.The Plant journal : for cell and molecular biology · 2025Article
- Broad application of the ALFA tagging technology for in planta nanobody-based imaging and biochemical characterization of plant proteins.Plant communications · 2025Article
- Iron limitation-induced endophytic Ammoniphilus assemblage promotes root apoplastic iron remobilization by attenuation of salicylic acid pathways.NPJ biofilms and microbiomes · 2025Article
- The transcription factor MYB30 promotes iron homeostasis by maintaining the stability of the FIT transcription factor.The Plant cell · 2025Article
- Barriers and carriers for transition metal homeostasis in plants.Plant communications · 2025Review
- Untangling iron threads: A deep dive into plant intracellular pools.Quantitative plant biology · 2025Review
- Quantitative tools for analyzing rhizosphere pH dynamics: localized and integrated approaches.Biology methods & protocols · 2025Article
- The receptor-like cytoplasmic kinase OsBSK1-2 regulates immunity via an HLH/bHLH complex.Journal of integrative plant biology · 2024Article
- Imaging of labile Fe2+ and Fe3+ in living Arabidopsis thaliana roots.Plant physiology · 2024Article
- Soil and Mineral Nutrients in Plant Health: A Prospective Study of Iron and Phosphorus in the Growth and Development of Plants.Current issues in molecular biology · 2024Review
- A major role of coumarin-dependent ferric iron reduction in strategy I-type iron acquisition in Arabidopsis.The Plant cell · 2024Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, iron uptake from the soil is tightly regulated to ensure optimal growth and development. Iron absorption in Arabidopsis root epidermal cells requires the IRT1 transporter that also allows the entry of certain non-iron metals, such as Zn, Mn, and Co. Recent work demonstrated that IRT1 endocytosis and degradation are controlled by IRT1 non-iron metal substrates in a ubiquitin-dependent manner. To better understand how metal uptake is regulated, we identified IRT1-interacting proteins in Arabidopsis roots by mass spectrometry and established an interactome of IRT1. Interestingly, the AHA2 proton pump and the FRO2 reductase, both of which work in concert with IRT1 in the acidification-reduction-transport strategy of iron uptake, were part of this interactome. We confirmed that IRT1, FRO2, and AHA2 associate through co-immunopurification and split-ubiquitin analyses, and uncovered that they form tripartite direct interactions. We characterized the dynamics of the iron uptake complex and showed that FRO2 and AHA2 ubiquitination is independent of the non-iron metal substrates transported by IRT1. In addition, FRO2 and AHA2 are not largely endocytosed in response to non-iron metal excess, unlike IRT1. Indeed, we provide evidence that the phosphorylation of IRT1 in response to high levels of non-iron metals likely triggers dissociation of the complex. Overall, we propose that a dedicated iron-acquisition protein complex exists at the cell surface of Arabidopsis root epidermal cells to optimize iron uptake.
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