Evidence map›Paper›PMID 32871749›Full record

ArticleIn vivo (Athens, Greece)

Reverse Transcriptase Affects Gametogenesis and Preimplantation Development in Mouse.

Chrysoula Kitsou, Leandros Lazaros, Alexandra Papoudou-Bai, Prodromos Sakaloglou, Eirini Mastora, Theodoros Lykovardakis, Katerina Giaka, Georgios Vartholomatos, Ioanna Bouba, Sofia Markoula and 2 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Chrysoula KitsouLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Leandros LazarosLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Alexandra Papoudou-BaiDepartment of Pathology, Ioannina University Hospital, Ioannina, Greece.
Prodromos SakaloglouLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Eirini MastoraLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Theodoros LykovardakisLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Katerina GiakaLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Georgios VartholomatosLaboratory of Hematology, Molecular Biology Unit, Ioannina University Hospital, Ioannina, Greece.
Ioanna BoubaLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece.
Sofia MarkoulaDepartment of Neurology, Ioannina University Hospital, Ioannina, Greece.
Anna BatistatouDepartment of Pathology, Ioannina University Hospital, Ioannina, Greece.
Ioannis GeorgiouLaboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece igeorgio@uoi.gr.
University of Ioannina · GRUniversity Hospital of Ioannina · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimThe expression of reverse transcriptase (RT) in ovaries, testes, gametes and embryos highlights its critical role in cell growth and differentiation. We sought to investigate the effects of the potent RT inhibitor lamivudine in gametogenesis and mouse embryo preimplantation development. MATERIALS AND

methodsMale and female FVB/N mice were treated with the reverse transcriptase inhibitor Lamivudine for seven consecutive weeks. Following treatment, mouse sperm parameters, testicular and ovarian morphology as well as post-IVF embryo development were evaluated.

resultsLamivudine impaired the sperm parameters and the testicular structure in male mice, the number of primordial germ cells and primary oocytes in ovaries of female mice, and the embryos' morphology and development up to the blastocyst stage during in vitro culture.

conclusionThe administration of lamivudine affected the processes of spermatogenesis and oogenesis as well as the in vitro preimplantation development of mouse embryos.

Indexed as

OocytesRNA-Directed DNA PolymeraseAnimalsBlastocystEmbryonic DevelopmentFemaleMaleMiceOogenesisReverse Transcriptase Polymerase Chain ReactionRNA-Directed DNA Polymeraseantiretroviral drugsembryogenesisLamivudineoogenesisreverse transcriptasespermatogenesis

Identifiers

PMID32871749
PMCPMC7652508
OpenAlexW3082587713

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.