Evidence map›Paper›PMID 32871378›Full record

ReviewJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2020

Affinity chromatography: A review of trends and developments over the past 50 years.

Elliott L Rodriguez, Saumen Poddar, Sazia Iftekhar, Kyungah Suh, Ashley G Woolfork, Susan Ovbude, Allegra Pekarek, Morgan Walters, Shae Lott, David S Hage

Abstract readHistorical ArticleReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Methods for the Investigation of Protein-Ligands Interactions.Advances in experimental medicine and biology · 2026
    Review
  9. Article
  10. Article
  11. Modulation of Aging Diseases via RAGE Targets: A Dietary Intervention Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  12. [Evaluation of Gradient-Variant Quantum Dot Fluorescence Technology for Rapid Detection ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025
    Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Elliott L RodriguezDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Saumen PoddarDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Sazia IftekharDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Kyungah SuhDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Ashley G WoolforkDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Susan OvbudeDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Allegra PekarekDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Morgan WaltersDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
Shae LottDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA.
David S HageDepartment of Chemistry, University of Nebraska, Lincoln, NE 68588, USA. Electronic address: dhage1@unl.edu.

Funding

CHROMATOGRAPHIC STUDIES OF FUNCTIONAL PROTEOMICSR01DK069629 · NIDDK · UNIVERSITY OF NEBRASKA LINCOLN · PI HAGE, DAVID S · 2006 to 2017
$1.8M
NIDDK NIH HHS R01 DK069629
6 · The paper itself

Abstract

The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968. This review examines the major developments and trends that have occurred in this technique over the past five decades. The basic principles and history of this area are first discussed. This is followed by an overview of the various supports, immobilization strategies, and types of binding agents that have been used in this field. The general types of applications and fields of use that have appeared for affinity chromatography are also considered. A survey of the literature is used to identify major trends in these topics and important areas of use for affinity chromatography in the separation, analysis, or characterization of chemicals and biochemicals.

Indexed as

Chromatography, AffinityBiochemistryBiomedical ResearchBiotechnologyHistory, 20th CenturyHistory, 21st CenturyHumansAffinity chromatographyAffinity ligandsAffinity supportsApplicationsImmobilization methods

Identifiers

PMID32871378
PMCPMC7584770

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.