Evidence map›Paper›PMID 32870455›Full record

ArticleJournal of fluorescence2020

A New Endoplasmic Reticulum (ER)-Targeting Fluorescent Probe for the Imaging of Cysteine in Living Cells.

Lei Zhou, Yunxia Li, Aiqin Zhou, Guanghui Zhang, Zhi-Qiang Cheng, Yong-Xi Ge, Shan-Kui Liu, Ricardo Bentes Azevedo, Juan Zhang, Shenglin Jiang and 1 more

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lei ZhouSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
Yunxia LiLaiyu Chemical Co., Itd, Laizhou, China.
Aiqin ZhouCollege of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China.
Guanghui ZhangLaiyu Chemical Co., Itd, Laizhou, China.
Zhi-Qiang ChengSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
Yong-Xi GeSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
Shan-Kui LiuSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
Ricardo Bentes AzevedoInstitute of Biological Sciences, University of Brasília, Brasilia, 70910900, Brazil.
Juan ZhangSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, China. bio_zhangj@ujn.edu.cn.
Shenglin JiangLaiyu Chemical Co., Itd, Laizhou, China. jsl@laiyu.com.
Cheng-Shi JiangSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, China. bio_jiangcs@ujn.edu.cn.ORCID http://orcid.org/0000-0002-2760-7596

Funding

Agricultural Variety Improvement Project of Shandong Province 2019LZGC007Doctoral Fund Project of University of Jinan 301/160100394National Major Science and Technology Projects of China (CN) 2019JZZY011116National Natural Science Foundation of China 21672082Natural Science Foundation of Shandong Province ZR2017BC101Natural Science Foundation of Shandong Province ZR2019YQ31Science and Technology Project of University of Jinan XKY2004
6 · The paper itself

Abstract

Cysteine (Cys) is an important endogenous amino acid and plays critical physiological roles in living systems. Herein, an endoplasmic reticulum (ER)-targeting fluorescent probe (FER-Cys) was designed and prepared for imaging of Cys in living cells. The probe FER-Cys consists of a fluorescein framework as the fluorescent platform, acrylate group as the response site for the selective recognition of Cys, and ER-specific p-toluenesulfonamide fragment. After the response of probe FER-Cys to Cys, a turn-on fluorescence signal at 546 nm could be detected obviously. The probe FER-Cys further shows desirable selectivity to Cys. Finally, the probe FER-Cys was proven to selectively detect Cys in live cells and successfully image the changes of Cys level in the cell models of H

Indexed as

Cell SurvivalCysteineEndoplasmic ReticulumFluorescent DyesHeLa CellsHumansHydrogen PeroxideOxidation-ReductionTolueneCysteineFluorescent DyesHydrogen PeroxideTolueneEndoplasmic reticulum-targetingFluorescent probeImaging of cysteineRedox imbalanceSelective recognition

Identifiers

PMID32870455

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.