Evidence map›Paper›PMID 32870453›Full record

ArticleJournal of fluorescence2020

Changes in Autofluorescence Level of Live and Dead Cells for Mouse Cell Lines.

Anastasiia A Kozlova, Roman A Verkhovskii, Alexey V Ermakov, Daniil N Bratashov

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
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  4. Tissue elasticity modulates cardiac pacemaker cell automaticity.American journal of physiology. Heart and circulatory physiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anastasiia A KozlovaSaratov State University, Saratov, Russia. anastasia.kozlova245@yandex.ru.ORCID https://orcid.org/0000-0002-9336-0317
Roman A VerkhovskiiSaratov State University, Saratov, Russia.ORCID https://orcid.org/0000-0003-1830-4582
Alexey V ErmakovSaratov State University, Saratov, Russia.ORCID https://orcid.org/0000-0001-8105-5932
Daniil N BratashovSaratov State University, Saratov, Russia.ORCID https://orcid.org/0000-0002-2096-4478

Funding

Russian Science Foundation 18-19-00354
6 · The paper itself

Abstract

Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells' metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 - macrophages, L929 - fibroblasts, C2C12 - myoblasts, and B16-F10 - melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.

Indexed as

Cytological TechniquesFluorescenceAnimalsCell LineCell SurvivalMiceCancer cell line autofluorescenceCell autofluorescenceDead cell autofluorescenceLive-cell autofluorescenceMouse cell lineMouse cell line autofluorescence

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.