ArticlePlanta2020
An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector.
Article in Planta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends.Molecular biology reports · 2026Review
- Dynamic chromatin accessibility reveals BrKAN2 as a key regulator of Chinese cabbage leaf heading.Molecular horticulture · 2026Article
- APathogens (Basel, Switzerland) · 2025Article
- Review
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- Function diversification of CONSTANS-like genes in Pyrus and regulatory mechanisms in response to different light quality.BMC plant biology · 2025Article
- Unraveling yield heterosis in Chinese cabbage hybrid by comparative transcriptomic analysis andFrontiers in plant science · 2025Article
- Geminiviruses and Food Security: A Molecular Genetics Perspective for Sustainable Agriculture in Africa.Plants (Basel, Switzerland) · 2024Review
- Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.Plant methods · 2024Article
- Glutamate Receptor-like (GLR) Family inInternational journal of molecular sciences · 2024Article
- Genome-Wide Identification of the U-Box E3 Ubiquitin Ligase Gene Family in Cabbage (Plants (Basel, Switzerland) · 2023Article
- Establishment of a Virus-Induced Gene-Silencing (VIGS) System in Tea Plant and Its Use in the Functional Analysis ofInternational journal of molecular sciences · 2022Article
- Geminivirus-Derived Vectors as Tools for Functional Genomics.Frontiers in microbiology · 2022Review
- Genome-wide characterization and analysis of the anthocyanin biosynthetic genes in Brassica oleracea.Planta · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
MAIN
conclusionCaLCuV-based VIGS effectively works in cabbage and contributes to efficient functional genomics research in Brassica crop species. Virus-induced gene silencing (VIGS), a posttranscriptional gene silencing method, is an effective technique for analysing the functions of genes in plants. However, no VIGS vectors have been available for Brassica oleracea until now. Here, tobacco rattle virus (TRV), pTYs and cabbage leaf curl virus (CaLCuV) gene-silencing vectors (PCVA/PCVB) were chosen to improve the VIGS system in cabbage using the phytoene desaturase (PDS) gene as an efficient visual indicator of VIGS. We successfully silenced the expression of PDS and observed photobleaching phenomena in cabbage in response to pTYs and CaLCuV, with the latter being more easy to operate and less expensive. The parameters potentially affecting the silencing efficiency of VIGS by CaLCuV in cabbage, including the targeting fragment strategy, inoculation method and incubation temperature, were then compared. The optimized CaLCuV-based VIGS system involves the following: an approximately 500 bp insert sequence, an Agrobacterium OD
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