ArticleInvestigative ophthalmology & visual science2020
Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea.
Article in Investigative ophthalmology & visual science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Temporal Variations in Inflammatory-Related Ocular Surface Parameters: A Systematic Review and Meta-Analysis.Investigative ophthalmology & visual science · 2026Pooled it
- Corneal epithelial cells upregulate macropinocytosis to engulf metabolically active axonal mitochondria released by injured axons.The ocular surface · 2025Article
- Mechanisms Regulating Mitochondrial Transfer in Human Corneal Epithelial Cells.Investigative ophthalmology & visual science · 2024Article
- Single-Cell Transcriptomic Analysis Reveals Dynamic Cellular Processes in Corneal Epithelium During Wound Healing in Cynomolgus Monkeys.Investigative ophthalmology & visual science · 2024Article
- Transient Receptor Potential Vanilloid-1 Channels Facilitate Axonal Degeneration of Corneal Sensory Nerves in Dry Eye.The American journal of pathology · 2024Article
- Diabetic Ocular Surface Has Defects in Oxygen Uptake Revealed by Optic Fiber Microsensor.Investigative ophthalmology & visual science · 2024Article
- Single cell analysis of short-term dry eye induced changes in cornea immune cell populations.Frontiers in medicine · 2024Article
- Optic Fiber Microsensor Reveals Specific Spatiotemporal Oxygen Uptake Profiles at the Mammalian Ocular Surface.Biosensors · 2023Article
- Molecular mechanisms regulating wound repair: Evidence for paracrine signaling from corneal epithelial cells to fibroblasts and immune cells following transient epithelial cell treatment with Mitomycin C.Experimental eye research · 2023Article
- High-fat intake reshapes the circadian transcriptome profile and metabolism in murine meibomian glands.Frontiers in nutrition · 2023Article
- Corneal regeneration: insights in epithelial stem cell heterogeneity and dynamics.Current opinion in genetics & development · 2022Review
- Short-Term High Fructose Intake Impairs Diurnal Oscillations in the Murine Cornea.Investigative ophthalmology & visual science · 2021Article
- Parity Attenuates Intraepithelial Corneal Sensory Nerve Loss in Female Mice.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: The circadian clock plays an important role in the expression and regulation of various genes and cellular processes in the body. Here, we study diurnal regulation of the growth and shedding of the sensory axons in the mouse cornea. Methods: Male and female BALB/cN mice were euthanized 90 minutes before and after the lights are turned on and off; at 5:30 AM, 8:30 AM, 5:30 PM, and 8:30 PM. Nerve terminal growth, shedding and overall axon density were assessed at these four time points using confocal imaging after staining axons in en face whole mount corneas with antibodies against βIII tubulin, GAP43, and L1CAM. In addition, corneal epithelial cell proliferation, thickness, and desquamation were assessed using ki67, LAMP1, Involucrin, and ZO1. Results: Nerve terminal shedding took place between 5:30 AM and 8:30 AM and correlated positively with the timing of apical cell desquamation. After shedding the tips of the nerve terminals, axonal growth increased as indicated by increased axonal GAP43 expression. At 5:30 PM and 8:30 PM before and after the lights are turned off, cell proliferation was reduced, and epithelial thickness was maximal. Conclusions: Intraepithelial corneal nerve growth and shedding are under diurnal control regulated by the time of day and whether lights are on or off. Axons extend during the day and are shed within 90 minutes after lights are turned on. The data presented in this article shed light on the potential role that circadian clock plays in corneal pain and discomfort.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.