ArticleApplied biochemistry and biotechnology2021
New Proluciferin Substrates for Human CYP4 Family Enzymes.
Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- The Enzyme-Bag Platform for Cytochrome P450 Biotransformation and High-Throughput Drug Metabolism Screening.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Orphan Cytochromes P450 as Possible Pharmacological Targets or Biomarkers in Breast Cancer.Current issues in molecular biology · 2025Review
- Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilizedSynthetic and systems biotechnology · 2024Article
- A facile strategy for the construction of a phage display cyclic peptide library for the selection of functional macrocycles.Chemical science · 2024Article
- Identification of New Substrates and Inhibitors of Human CYP2A7.Molecules (Basel, Switzerland) · 2024Article
- Mutual Influence of Human Cytochrome P450 Enzymes and UDP-Glucuronosyltransferases on Their Respective Activities in Recombinant Fission Yeast.Biomedicines · 2023Article
- WGCNA combined with GSVA to explore biomarkers of refractory neocortical epilepsy.IBRO neuroscience reports · 2022Article
- New luciferin-based probe substrates for human CYP26A1.Biochemistry and biophysics reports · 2020Article
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the synthesis of seven new proluciferins for convenient activity determination of enzymes belonging to the cytochrome P450 (CYP) 4 family. Biotransformation of these probe substrates was monitored using each of the twelve human CYP4 family members, and eight were found to act at least on one of them. For all substrates, activity of CYP4Z1 was always highest, while that of CYP4F8 was always second highest. Site of metabolism (SOM) predictions involving SMARTCyp and docking experiments helped to rationalize the observed activity trends linked to substrate accessibility and reactivity. We further report the first homology model of CYP4F8 including suggested substrate recognition residues in a catalytically competent conformation accessed by replica exchange solute tempering (REST) simulations.
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