Evidence map›Paper›PMID 32869030›Full record

ArticlebioRxiv : the preprint server for biology2020

A single immunization with spike-functionalized ferritin vaccines elicits neutralizing antibody responses against SARS-CoV-2 in mice.

Abigail E Powell, Kaiming Zhang, Mrinmoy Sanyal, Shaogeng Tang, Payton A Weidenbacher, Shanshan Li, Tho D Pham, John E Pak, Wah Chiu, Peter S Kim

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Abigail E PowellDepartment of Biochemistry & Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Kaiming ZhangDepartment of Bioengineering & James H. Clark Center, Stanford University, Stanford, CA 94305, USA.
Mrinmoy SanyalDepartment of Biochemistry & Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Shaogeng TangDepartment of Biochemistry & Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Payton A WeidenbacherDepartment of Biochemistry & Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Shanshan LiDepartment of Bioengineering & James H. Clark Center, Stanford University, Stanford, CA 94305, USA.
Tho D PhamDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
John E PakChan Zuckerberg Biohub, San Francisco, CA 94158, USA.
Wah ChiuDepartment of Bioengineering & James H. Clark Center, Stanford University, Stanford, CA 94305, USA.
Peter S KimDepartment of Biochemistry & Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Stanford University · USChan Zuckerberg Initiative (United States) · USSLAC National Accelerator Laboratory · USStanford Blood Center · US

Funding

Virology BSL4 CoreP01AI120943 · NIAID · WASHINGTON UNIVERSITY · PI Gaya K. Amarasinghe · 2016 to 2026
$30.2M
3 D ELECTRON MICROSCOPY OF MACROMOLECULES (5P41RR002250-27)P41GM103832 · NIGMS · STANFORD UNIVERSITY · PI CHIU, WAH · 2012 to 2019
$15.1M
Molecular Interactions of HIV-1 with the Nuclear Pore ComplexR01AI148382 · NIAID · EMORY UNIVERSITY · PI MELIKIAN, GREGORY B, SARAFIANOS, STEFAN G · 2019 to 2022
$5.5M
Stem cell, tumor and bone marrow microenvironment cross-talk in vivoDP2OD002160 · OD · UNIVERSITY OF CHICAGO · PI SIPKINS, DOROTHY A · 2007 to 2007
$2.3M
NIAID NIH HHS P01 AI120943NIAID NIH HHS R01 AI148382NIGMS NIH HHS P41 GM103832NIH HHS DP2 OD002160
6 · The paper itself

Abstract

Development of a safe and effective SARS-CoV-2 vaccine is a public health priority. We designed subunit vaccine candidates using self-assembling ferritin nanoparticles displaying one of two multimerized SARS-CoV-2 spikes: full-length ectodomain (S-Fer) or a C-terminal 70 amino-acid deletion (SΔC-Fer). Ferritin is an attractive nanoparticle platform for production of vaccines and ferritin-based vaccines have been investigated in humans in two separate clinical trials. We confirmed proper folding and antigenicity of spike on the surface of ferritin by cryo-EM and binding to conformation-specific monoclonal antibodies. After a single immunization of mice with either of the two spike ferritin particles, a lentiviral SARS-CoV-2 pseudovirus assay revealed mean neutralizing antibody titers at least 2-fold greater than those in convalescent plasma from COVID-19 patients. Additionally, a single dose of SΔC-Fer elicited significantly higher neutralizing responses as compared to immunization with the spike receptor binding domain (RBD) monomer or spike ectodomain trimer alone. After a second dose, mice immunized with SΔC-Fer exhibited higher neutralizing titers than all other groups. Taken together, these results demonstrate that multivalent presentation of SARS-CoV-2 spike on ferritin can notably enhance elicitation of neutralizing antibodies, thus constituting a viable strategy for single-dose vaccination against COVID-19.

Identifiers

PMID32869030
PMCPMC7457616
OpenAlexW3082610737

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.