ArticleScientific reports2020
Demystifying biotrophs: FISHing for mRNAs to decipher plant and algal pathogen-host interaction at the single cell level.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Local endoreduplication of the host is a conserved process during Phytomyxea-host interaction.Frontiers in microbiology · 2024Article
- Multiple transcription factors involved in the response of Chinese cabbage againstFrontiers in plant science · 2024Article
- Host-Parasite interaction between brown algae and eukaryote biotrophic pathogens.Current research in microbial sciences · 2024Article
- Phagocytosis underpins the biotrophic lifestyle of intracellular parasites in the class Phytomyxea (Rhizaria).The New phytologist · 2023Article
- The clubroot pathogen Plasmodiophora brassicae: A profile update.Molecular plant pathology · 2023Review
- What Can We Learn from -Omics Approaches to Understand Clubroot Disease?International journal of molecular sciences · 2022Review
- Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-FluorescenceMicroorganisms · 2021Article
- Molecular Pathotyping ofPathogens (Basel, Switzerland) · 2021Review
- Candidate Effectors ofFrontiers in microbiology · 2021Article
- Endomembrane-TargetingFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Plant-pathogen interactions follow spatial and temporal developmental dynamics where gene expression in pathogen and host undergo crucial changes. Therefore, it is of great interest to detect, quantify and localise where and when key genes are active to understand these processes. Many pathosystems are not accessible for genetic amendments or other spatially-resolved gene expression monitoring methods. Here, we adapt single molecule FISH techniques to demonstrate the presence and activity of mRNAs at the single-cell level using phytomyxids in their plant and algal host in lab and field material. This allowed us to monitor and quantify the expression of genes from the clubroot pathogen Plasmodiophora brassicae, several species of its Brassica hosts, and of several brown algae, including the genome model Ectocarpus siliculosus, infected with the phytomyxid Maullinia ectocarpii. We show that mRNAs are localised along a spatiotemporal gradient, thus providing a proof-of-concept of the usefulness of single-molecule FISH to increase knowledge about the interactions between plants, algae and phytomyxids. The methods used are easily applicable to any interaction between microbes and their algal or plant host, and have therefore the potential to rapidly increase our understanding of key, spatially- and temporally-resolved processes underpinning complex plant-microbe interactions.
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Registered trials
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