ArticlePoultry science2020
The effect of total flavonoids of Epimedium on granulosa cell development in laying hens.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Heterogeneous CRISPR/Cas9 Editing ofInternational journal of molecular sciences · 2026Article
- Dietary supplementation with Cuscuta chinensis improves ovarian function and production performance in aged laying hens via modulation of steroid hormone biosynthesis and gut microbiota.Poultry science · 2026Article
- Protective Effects ofAnimals : an open access journal from MDPI · 2026Article
- Soybean isoflavones enhance production and albumen quality in aged laying hens by modulating ovarian function, immunity, and antioxidant capacity.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Effect of complex fermentation of two lactic acid bacteria on antioxidant activity, hypoglycaemic activity and metabolomics of corn juice.Food science and biotechnology · 2025Article
- Tartary Buckwheat Flavonoids and 25-Hydroxyvitamin DAnimals : an open access journal from MDPI · 2025Article
- Effects of seabuckthorn pomace on rumen development, intramuscular fatty acids and antioxidant capacity in weaned lambs.Frontiers in veterinary science · 2025Article
- Dietary supplementation withFrontiers in veterinary science · 2024Article
- Effects of dietary pretreated Chinese herbal medicine supplementation on production performance, egg quality, uterine histopathological changes, and antioxidant capacity in late-phase laying hens.Frontiers in physiology · 2023Article
- Effects of Total Flavonoids ofAntioxidants (Basel, Switzerland) · 2022Article
- AnFrontiers in veterinary science · 2022Article
- Evaluating the effect of lactic acid bacteria fermentation on quality, aroma, and metabolites of chickpea milk.Frontiers in nutrition · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the impact of total flavonoids of Epimedium (TFE) on the development of follicles of laying hens, 3 types of follicles including primary, prehierarchical, and preovulatory follicles were selected to obtain the follicular granulosa cells cultured in vitro. First, extraction of TFE was conducted by alcohol-soluble and ultrasonic methods. The effects of TFE on activity and proliferation of follicular granulosa cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and measuring the expression of proliferating cell nuclear antigen mRNA through real-time quantitative polymerase chain reaction, and the expression of the follicle-stimulating hormone receptor, luteinizing hormone receptor, steroidogenic acute regulatory protein, and cytochrome P450 family 11 subfamily A member 1 mRNA was detected to study the functions of TFE affecting the differentiation and hormone secretion by granulosa cells. The results showed that TFE significantly improved the proliferation of 3 types of granulosa cells and promoted the differentiation of granulosa cells and accelerated the conversion of primary follicles to prehierarchical follicles. Total flavonoids of Epimedium played an important role in promoting progesterone secretion by prehierarchical and preovulatory granulosa cells. The results indicated that TFE could promote proliferation and differentiation of follicular granulosa cells and improve hormone secretion and follicle development, which provided reference data for TFE used as a feed additive or safe Chinese veterinary medicine to promote the laying rate.
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