Evidence map›Paper›PMID 32866936›Full record

ArticleTranslational oncology2020

Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules.

Hannah M Hollandsworth, Verena Schmitt, Siamak Amirfakhri, Filemoni Filemoni, Alexej Schmidt, Maréne Landström, Mykola Lyndin, Steffen Backert, Markus Gerhard, Gunther Wennemuth and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational oncology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. [Diagnostic Value of Serum Pepsinogen Ⅰ/Pepsinogen Ⅱ Combined with Tumor Markers forSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023
    Article
  5. Effects ofFrontiers in immunology · 2023
    Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 4 countries.

Hannah M HollandsworthDepartment of Surgery, University of California, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA; VA San Diego Healthcare System, San Diego, CA, USA.
Verena SchmittInstitute of Anatomy, University Hospital, University Duisburg-Essen, Essen, Germany.
Siamak AmirfakhriDepartment of Surgery, University of California, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA; VA San Diego Healthcare System, San Diego, CA, USA.
Filemoni FilemoniDepartment of Surgery, University of California, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA; VA San Diego Healthcare System, San Diego, CA, USA.
Alexej SchmidtMedical Biosciences, Umeå University, Umeå, Sweden.
Maréne LandströmMedical Biosciences, Umeå University, Umeå, Sweden.
Mykola LyndinDepartment of Pathology, Sumy State University, Sumy, Ukraine.
Steffen BackertDepartment of Biology, Division of Microbiology, Friedrich Alexander University Erlangen, Erlangen, Germany.
Markus GerhardInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany.
Gunther WennemuthInstitute of Anatomy, University Hospital, University Duisburg-Essen, Essen, Germany.
Robert M HoffmanDepartment of Surgery, University of California, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA; VA San Diego Healthcare System, San Diego, CA, USA; AntiCancer, Inc., San Diego, CA, USA.
Bernhard B SingerInstitute of Anatomy, University Hospital, University Duisburg-Essen, Essen, Germany.
Michael BouvetDepartment of Surgery, University of California, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA; VA San Diego Healthcare System, San Diego, CA, USA. Electronic address: mbouvet@ucsd.edu.
University of Duisburg-Essen · DEVA San Diego Healthcare System · USUmeå University · SEUniversity of California, San Diego · USFriedrich-Alexander-Universität Erlangen-Nürnberg · DEInstitute of Medical Microbiology and Hygiene · DESumy State University · UA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

HopQ is an outer-membrane protein of Helicobacter pylori that binds to human carcinoembryonic antigen-related cell-adhesion molecules (CEACAMs) with high specificity. We aimed to investigate fluorescence targeting of CEACAM-expressing colorectal tumors in patient-derived orthotopic xenograft (PDOX) models with fluorescently labeled recombinant HopQ (rHopQ). Western blotting, flow cytometry and ELISA were performed to determine the efficiency of rHopQ binding to CEACAMs. rHopQ was conjugated to IR800DyeCW (rHopQ-IR800). Nude mice received orthotopic implantation of colon cancer tumors. Three weeks later, mice were administered 25 μg or 50 μg HopQ-IR800 and imaged 24 or 48 h later. Intravital images were analyzed for tumor-to-background ratio (TBR). Flow cytometry and ELISA demonstrated binding of HopQ to CEACAM1, 3 and 5. Dose-response intravital imaging in PDOX models demonstrated optimal results 48 h after administration of 50 μg rHopQ-IR800 (TBR = 3.576) in our protocol. Orthotopic models demonstrated clear tumor margins of primary tumors and small regional metastases with a mean TBR = 3.678 (SD ± 1.027). rHopQ showed specific binding to various CEACAMs in PDOX models. rHopQ may be useful for CEACAM-positive tumor and metastasis detection for pre-surgical diagnosis, intra-operative imaging and fluorescence-guided surgery.

Identifiers

PMID32866936
PMCPMC7475271
OpenAlexW3081757633

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.