ArticlePLoS pathogens2020
Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Review
- Pathogen-induced rerouting of host membrane trafficking.Current opinion in cell biology · 2025Review
- The bacterial type three secretion system induces mechanoporation of vacuolar membranes.PLoS biology · 2025Article
- TheVirulence · 2024Article
- Role of Rab GTPases in Bacteria Escaping from Vesicle Trafficking of Host Cells.Journal of microbiology (Seoul, Korea) · 2024Review
- Microtubules provide force to promote membrane uncoating in vacuolar escape for a cyto-invasive bacterial pathogen.Nature communications · 2024Article
- Orientia tsutsugamushi: A life between escapes.MicrobiologyOpen · 2023Article
- The cell wall lipoprotein CD1687 acts as a DNA binding protein during deoxycholate-induced biofilm formation in Clostridioides difficile.NPJ biofilms and microbiomes · 2023Article
- In through the Out Exit: the Role of the Exocyst in Listeria monocytogenes Cell Entry.Infection and immunity · 2022Article
- The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence.The Plant cell · 2022Article
- Live imaging of Yersinia translocon formation and immune recognition in host cells.PLoS pathogens · 2022Article
- Modification of phosphoinositides by theFrontiers in cellular and infection microbiology · 2022Review
- CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection ofFrontiers in bioengineering and biotechnology · 2022Article
- Purification of infection-associated macropinosomes by magnetic isolation for proteomic characterization.Nature protocols · 2021Review
- New methods to decrypt emerging macropinosome functions during the host-pathogen crosstalk.Cellular microbiology · 2021Review
- Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized byFrontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella flexneri invades host cells by entering within a bacteria-containing vacuole (BCV). In order to establish its niche in the host cytosol, the bacterium ruptures its BCV. Contacts between S. flexneri BCV and infection-associated macropinosomes (IAMs) formed in situ have been reported to enhance BCV disintegration. The mechanism underlying S. flexneri vacuolar escape remains however obscure. To decipher the molecular mechanism priming the communication between the IAMs and S. flexneri BCV, we performed mass spectrometry-based analysis of the magnetically purified IAMs from S. flexneri-infected cells. While proteins involved in host recycling and exocytic pathways were significantly enriched at the IAMs, we demonstrate more precisely that the S. flexneri type III effector protein IpgD mediates the recruitment of the exocyst to the IAMs through the Rab8/Rab11 pathway. This recruitment results in IAM clustering around S. flexneri BCV. More importantly, we reveal that IAM clustering subsequently facilitates an IAM-mediated unwrapping of the ruptured vacuole membranes from S. flexneri, enabling the naked bacterium to be ready for intercellular spread via actin-based motility. Taken together, our work untangles the molecular cascade of S. flexneri-driven host trafficking subversion at IAMs to develop its cytosolic lifestyle, a crucial step en route for infection progression at cellular and tissue level.
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