Evidence map›Paper›PMID 32862756›Full record

ArticleThe Journal of international medical research2020

Influence of different erythrocyte storage times on the macrophage response in haemorrhagic shock mice.

Zhen-Zhou Li, Xiao-Xiao Wang, Li Ma, Huan Wang, Jia-Ming Xu, Xiao-Fang Zhou, Yang Liu, Jian-Rong Guo

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of international medical research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Retraction.The Journal of international medical research · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Zhen-Zhou LiDepartment of Anaesthesiology, General Hospital of Ningxia Medical University, Yinchuan, China.
Xiao-Xiao WangDepartment of Anaesthesiology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai, China.
Li MaDepartment of Anaesthesiology, Shanghai Pudong New Area People's Hospital, Shanghai, China.
Huan WangDepartment of Anaesthesiology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai, China.
Jia-Ming XuDepartment of Anaesthesiology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai, China.
Xiao-Fang ZhouDepartment of Anaesthesiology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai, China.
Yang LiuDepartment of Anaesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Jian-Rong GuoDepartment of Anaesthesiology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai, China.ORCID https://orcid.org/0000-0002-1523-6273
Second Military Medical University · CNPudong New Area People's Hospital · CNTongji University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the characteristics of the macrophage response to transfusion of erythrocytes kept at different storage times in the mouse model of haemorrhagic shock.

methodsErythrocytes were isolated from mice and stored for 7, 21 or 35 days and samples injected intravenously into haemorrhagic shock mice. Changes in macrophages, inflammatory cytokines and T cell differentiation were assessed using flow cytometry or enzyme-linked immunosorbent assay (ELISA). In a second experiment, haemorrhagic shock mice were injected with 21D-erythrocytes and the expression of nuclear factor erythroid 2 p45-related factor 2 (Nrf2), arginine -1 (Arg-1) and inducible nitrous oxide (iNOS) determined.

resultsThe proportion of M1-polarized macrophages was greatest in the 21D group while M2 macrophages tended to increase with the erythrocyte storage time. Levels of inflammatory cytokines and T helper 1 (Th1) cells increased in proportion to erythrocytes storage time. Most regulatory T cells (Treg) were found at 21D. Arg-1 expression was significantly increased in a group that received an heme oxygenase 1 (HO-1) agonist and significantly decreased in a group that received an HO-1 inhibitor but there were no differences in the expression of iNOS or Nrf2.

conclusion21D storage time may be an important time point for erythrocyte storage and immunity response and Arg-1 may have a role in the macrophage response to erythrocyte infusion.

Indexed as

Shock, HemorrhagicAnimalsCytokinesErythrocytesHeme Oxygenase-1MacrophagesMiceNF-E2-Related Factor 2CytokinesHeme Oxygenase-1NF-E2-Related Factor 2Arginine-1Erythrocytes infusionHO-1immune responsemacrophage polarizationNrf2

Identifiers

PMID32862756
PMCPMC7469737
OpenAlexW3082650192

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.