Evidence map›Paper›PMID 32861143›Full record

ArticleVirology2020

Recombinant hemagglutinin glycoproteins provide insight into binding to host cells by H5 influenza viruses in wild and domestic birds.

Carmen Jerry, David Stallknecht, Christina Leyson, Roy Berghaus, Brian Jordan, Mary Pantin-Jackwood, Gavin Hitchener, Monique França

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Carmen JerryPoultry Diagnostic and Research Center, 953 College, Station Road, Athens, GA, 30605, USA; The Department of Pathology, College of Veterinary Medicine, 501 D.W. Brooks Drive, Athens, GA, 30602, USA.
David StallknechtSoutheastern Cooperative Wildlife Disease Study, 589 D.W Brooks Drive, Athens, GA, 30602, USA.
Christina LeysonSoutheast Poultry Research Laboratory, U.S. National Poultry Research Center, U.S. Dept. of Agriculture, Agricultural Research Service, 934 College Station Road, Athens, GA, 30605, USA.
Roy BerghausFood Animal Health and Management Program, Veterinary Medical Center, 2200 College Station Road, Athens, GA, 30602, USA.
Brian JordanPoultry Diagnostic and Research Center, 953 College, Station Road, Athens, GA, 30605, USA.
Mary Pantin-JackwoodSoutheast Poultry Research Laboratory, U.S. National Poultry Research Center, U.S. Dept. of Agriculture, Agricultural Research Service, 934 College Station Road, Athens, GA, 30605, USA.
Gavin HitchenerCornell University Duck Research Laboratory, 192 Old Country Road, Eastport, NY, 11941, USA.
Monique FrançaPoultry Diagnostic and Research Center, 953 College, Station Road, Athens, GA, 30605, USA. Electronic address: mfranca@uga.edu.
U.S. National Poultry Research Center · USUniversity of Georgia · USCornell University · US

Funding

NIAID NIH HHS HHSN272201400006C
6 · The paper itself

Abstract

Clade 2.3.4.4, H5 subtype highly pathogenic avian influenza viruses (HPAIVs) have caused devastating effects across wild and domestic bird populations. We investigated differences in the intensity and distribution of the hemagglutinin (HA) glycoprotein binding of a clade 2.3.4.4 H5 HPAIV compared to a H5 low pathogenic avian influenza virus (LPAIV). Recombinant HA from gene sequences from a HPAIV, A/Northern pintail/Washington/40964/2014(H5N2) and a LPAIV, A/mallard/MN/410/2000(H5N2) were generated and, via protein histochemistry, HA binding in respiratory, intestinal and cloacal bursal tissue was quantified as median area of binding (MAB). Poultry species, shorebirds, ducks and terrestrial birds were used. Differences in MAB were observed between the HPAIV and LPAIV H5 HAs. We demonstrate that clade 2.3.4.4 HPAIV H5 HA has a broader host cell binding across a variety of bird species compared to the LPAIV H5 HA. These findings support published results from experimental trials, and outcomes of natural disease outbreaks with these viruses.

Indexed as

AnimalsAnimals, DomesticAnimals, WildBursa of FabriciusCloacaDucksEaglesGene ExpressionHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H5N2 SubtypeInfluenza in BirdsIntestinal MucosaLungPoultryProtein BindingRecombinant Proteinshemagglutinin, avian influenza A virusHemagglutinin Glycoproteins, Influenza VirusRecombinant ProteinsH5N2HemagglutininHighly pathogenic avian influenza virusLow pathogenic avian influenza virusPoultryRecombinant proteinTerrestrial birdsTropismWaterfowl

Identifiers

PMID32861143
PMCPMC7554162
OpenAlexW3048675296

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.