ArticlePlant cell reports2020
Silencing AC1 of Tomato leaf curl virus using artificial microRNA confers resistance to leaf curl disease in transgenic tomato.
Article in Plant cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Metavirome Detection and Analysis of Viruses Present in Diseased Pumpkin in Shandong, China.Viruses · 2026Article
- amiR414e-mediated silencing confers resistance to Cotton leaf curl Multan virus in cotton.Molecular biology reports · 2026Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- Artificial miRNAs and target-mimics as potential tools for crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Advances in understanding plant-pathogen interactions: insights from tomato as a model system.Virusdisease · 2024Review
- Exogenous Application of dsRNA for Protection against Tomato Leaf Curl New Delhi Virus.Viruses · 2024Article
- Identification of pathogenicity determinants in ToLCNDV and their RNAi-based knockdown for disease management inFrontiers in microbiology · 2024Article
- A comprehensive review onFrontiers in genetics · 2024Review
- Advances and Prospects of Virus-Resistant Breeding in Tomatoes.International journal of molecular sciences · 2023Review
- Size variations of mesoporous silica nanoparticle control uptake efficiency and delivery of AC2-derived dsRNA for protection against tomato leaf curl New Delhi virus.Plant cell reports · 2023Article
- Small RNAs: Efficient and miraculous effectors that play key roles in plant-microbe interactions.Molecular plant pathology · 2023Review
- Article
- Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus.Frontiers in plant science · 2023Article
- Current status, breeding strategies and future prospects for managing chilli leaf curl virus disease and associated begomoviruses in Chilli (Frontiers in plant science · 2023Review
- DNA methylation dynamics in response to abiotic and pathogen stress in plants.Plant cell reports · 2022Review
- Regulation of small RNA-mediated high temperature stress responses in crop plants.Plant cell reports · 2022Review
- Artificial intelligence knacks-based stochastic paradigm to study the dynamics of plant virus propagation model with impact of seasonality and delays.European physical journal plus · 2022Article
- The sly-miR166-SlyHB module acts as a susceptibility factor during ToLCNDV infection.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
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Authors and funding
2 authors.
Funding
Abstract
key messageExpression of artificial microRNA targeting ATP binding domain of AC1 in transgenic tomato confers resistance to Tomato leaf curl disease without impacting the yield of tomato. Tomato curl leaf disease caused by Tomato leaf curl virus (ToLCV) is a key constraint to tomato cultivation worldwide. Engineering transgenic plants expressing artificial microRNAs (amiRNAs) against the AC1 gene of Tomato leaf curl New Delhi virus (ToLCNDV), which is important for virus replication and pathogenicity, would consequently confer virus resistance and reduce crop loss in the economically important crops. This study relates to an amiRNA developed on the sequence of Arabidopsis miRNA319a, targeting the ATP/GTP binding domain of AC1 gene of ToLCNDV. The AC1-amiR was found to regulate the abundance of AC1, providing an excellent strategy in providing defense against ToLCNDV. Transgenic lines over-expressing AC1-amiR, when challenged with ToLCNDV, showed reduced disease symptoms and high percentage resistance ranging between ∼ 40 and 80%. The yield of transgenic plants was significantly higher upon ToLCNDV infection as compared to the non-transgenic plants. Although the natural resistance resources against ToLCNDV are not available, this work streamlines a novel amiRNA-based mechanism that may have the potential to develop viral resistance strategies in tomato, apart from its normal symptom development properties as it is targeting the conserved region against which higher accumulation of small interfering RNAs (siRNA) occurred in a naturally tolerant tomato cultivar.
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