Evidence map›Paper›PMID 32859151›Full record

ArticleBMC genomics2020

Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo.

Somayeh Kordafshari, Pollob Shil, Marc S Marenda, Olusola M Olaogun, Barbara Konsak-Ilievski, Jillian Disint, Amir H Noormohammadi

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Virulence factors ofFrontiers in microbiology · 2022
    Article
  3. Comparative Genomics ofFrontiers in veterinary science · 2021
    Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Somayeh KordafshariAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia. somayeh.kordafshari@unimelb.edu.au.ORCID http://orcid.org/0000-0001-5084-1092
Pollob ShilAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.
Marc S MarendaAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.
Olusola M OlaogunAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.
Barbara Konsak-IlievskiAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.
Jillian DisintAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.
Amir H NoormohammadiAsia Pacific Centre for Animal Health, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.
The University of Melbourne · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGenomic comparison of Mycoplasma synoviae vaccine strain MS-H and the MS-H parental strain 86,079/7NS established a preliminary profile of genes related to attenuation of MS-H. In this study we aimed to identify the stability of mutations found in MS-H after passage in experimental or field chickens, and to evaluate if any reverse mutation may be associated with changes in characteristics of MS-H in vitro or in vivo.

resultsWhole genome sequence analysis of 5 selected MS-H field reisolates revealed that out of 32 mutations reported previously in MS-H, 28 remained stable, while four found to be reversible to the wild-type. Each isolate possessed mutations in one to three of the genes obg, oppF

conclusionsThese results suggest that the majority of the MS-H mutations are stable after passage in vaccinated chickens. Characterisation of stable mutations found in MS-H could be utilised to develop rapid diagnostic techniques for differentiation of vaccine from field strains or ts- MS-H reisolates.

Indexed as

Mycoplasma InfectionsMycoplasma synoviaePoultry DiseasesAnimalsBacterial ProteinsBacterial VaccinesChickensMutationBacterial ProteinsBacterial VaccinesGenomic comparisonMS-H field reisolatesMS-H vaccine strainMycoplasma synoviaeStable and unstable mutations

Identifiers

PMID32859151
PMCPMC7456371
OpenAlexW3082691693

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.