ArticleMethods and protocols2020
Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample.
Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
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- Functional gut variability drives metabolic and transcriptional features in neonatal piglets.NPJ science of food · 2025Article
- Maternal serum and placental metabolomes in association with prenatal exposure to per- and polyfluoroalkyl substances and their relevance to child neurodevelopment in an ASD-enriched cohort.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Dual Impact of Iron Deficiency and Antibiotics on Host Metabolism: A Tissue-Level Analysis.Metabolites · 2025Article
- The role of alpha-lactalbumin in modulating tryptophan metabolism and serotonin synthesis.NPJ science of food · 2025Article
- Placental Metabolism Is Linked to Prenatal Vitamin Supplement Use in the First Month of Pregnancy in the MARBLES Cohort.The Journal of nutrition · 2025Article
- Neurodevelopment and Metabolism in the Maternal-Placental-Fetal Unit.JAMA network open · 2024Article
- Calorie restriction and pravastatin administration during pregnancy in obese rhesus macaques modulates maternal and infant metabolism and infant brain and behavioral development.Frontiers in nutrition · 2023Article
- Multi-omic brain and behavioral correlates of cell-free fetal DNA methylation in macaque maternal obesity models.Nature communications · 2022Article
- Maternal Serum and Placental Metabolomes in Association with Prenatal Phthalate Exposure and Neurodevelopmental Outcomes in the MARBLES Cohort.Metabolites · 2022Article
- Impact of Maternal Obesity on the Gestational Metabolome and Infant Metabolome, Brain, and Behavioral Development in Rhesus Macaques.Metabolites · 2022Article
- Integrated Metabolomics and Transcriptomics Using an Optimised Dual Extraction Process to Study Human Brain Cancer Cells and Tissues.Metabolites · 2021Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.
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