Evidence map›Paper›PMID 32857953›Full record

ArticleMolecular cell2020

Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.

Ameya P Jalihal, Sethuramasundaram Pitchiaya, Lanbo Xiao, Pushpinder Bawa, Xia Jiang, Karan Bedi, Abhijit Parolia, Marcin Cieslik, Mats Ljungman, Arul M Chinnaiyan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed.

  1. Article
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  9. Divergent condensates tune transcriptional responses during stress.bioRxiv : the preprint server for biology · 2026
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  11. Article
  12. Review
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21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Ameya P JalihalSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA; Cell and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI 48109, USA.
Sethuramasundaram PitchiayaMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA; Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: sethu@umich.edu.
Lanbo XiaoMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
Pushpinder BawaMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA.
Xia JiangMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA.
Karan BediDepartment of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA.
Abhijit ParoliaMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
Marcin CieslikMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA.
Mats LjungmanDepartment of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA; Rogel Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA; Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, USA; Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
Arul M ChinnaiyanMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Urology, University of Michigan, Ann Arbor, MI 48109, USA; Rogel Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA; Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: arul@umich.edu.
Nils G WalterSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA; Rogel Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA; Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: nwalter@umich.edu.

Funding

Tissue/InformaticsP50CA186786 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ARUL M CHINNAIYAN, Ganesh S Palapattu · 2014 to 2026
$27.6M
TRAINING PROGRAM IN LUNG BIOLOGY AND PATHOBIOLOGYT32HL007829 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI DOLLY MEHTA · 1995 to 2026
$15.6M
CELLULAR AND MOLECULAR BIOLOGY AT MICHIGANT32GM007315 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PUTHENVEEDU, MANOJKUMAR A · 1985 to 2021
$12.8M
PI3K/PTEN/Akt signaling and the genesis of cancerR01CA090764 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI Nissim Hay · 2001 to 2026
$9.4M
The Role of Akt in Cell Survival and Cell GrowthR01AG016927 · NIA · UNIVERSITY OF ILLINOIS AT CHICAGO · PI HAY, NISSIM · 1998 to 2023
$9.0M
The RNA nanomachines of the gene expression machinery dissected at the single molecule levelR35GM131922 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NILS G WALTER · 2019 to 2026
$6.9M
Hexokinase 2 in liver cancerR01CA206167 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI HAY, NISSIM · 2016 to 2020
$2.1M
Timing and coordination of the conformational rearrangements mediating splicingR01GM122803 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WALTER, NILS G · 2017 to 2019
$880k
Elucidating the Mechanisms by which Hexokinase 2 Regulates Breast Cancer MetastasisF30CA228191 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI BLAHA, CATHERINE · 2018 to 2021
$195k
BLRD VA I01 BX000733BLRD VA IK6 BX004602Howard Hughes Medical InstituteNCI NIH HHS F30 CA228191NCI NIH HHS P50 CA186786NCI NIH HHS R01 CA090764NCI NIH HHS R01 CA206167NHLBI NIH HHS T32 HL007829NIA NIH HHS R01 AG016927NIGMS NIH HHS R01 GM122803NIGMS NIH HHS R35 GM131922NIGMS NIH HHS T32 GM007315
6 · The paper itself

Abstract

Processing bodies (PBs) and stress granules (SGs) are prominent examples of subcellular, membraneless compartments that are observed under physiological and stress conditions, respectively. We observe that the trimeric PB protein DCP1A rapidly (within ∼10 s) phase-separates in mammalian cells during hyperosmotic stress and dissolves upon isosmotic rescue (over ∼100 s) with minimal effect on cell viability even after multiple cycles of osmotic perturbation. Strikingly, this rapid intracellular hyperosmotic phase separation (HOPS) correlates with the degree of cell volume compression, distinct from SG assembly, and is exhibited broadly by homo-multimeric (valency ≥ 2) proteins across several cell types. Notably, HOPS sequesters pre-mRNA cleavage factor components from actively transcribing genomic loci, providing a mechanism for hyperosmolarity-induced global impairment of transcription termination. Our data suggest that the multimeric proteome rapidly responds to changes in hydration and molecular crowding, revealing an unexpected mode of globally programmed phase separation and sequestration.

Indexed as

Transcription Termination, GeneticAnimalsCell SizeCell SurvivalEndoribonucleasesHumansOsmotic PressureProteomeRNA PrecursorsStress, PhysiologicalTrans-ActivatorsDCP1A protein, humanEndoribonucleasesProteomeRNA PrecursorsTrans-Activatorscell volumehydrationhyperosmotic stressmolecular crowdingphase separationprocessing bodiesprotein multivalencyself-interactionstress granulesstress response

Identifiers

PMID32857953
PMCPMC7502480

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.